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N-乙酰半胱氨酸对慢性饮酒大鼠肺纤维化的干预作用的研究

王静宜1,于洪志2,3,武俊平1,杜钟珍4,吴琦1   

  1. 1. 天津市海河医院
    2. 天津市海河医院 天津市呼吸疾病研究所
    3.
    4. 天津海河医院
  • 收稿日期:2011-12-14 修回日期:2012-04-11 出版日期:2012-10-15 发布日期:2012-10-15
  • 通讯作者: 王静宜

  • Received:2011-12-14 Revised:2012-04-11 Published:2012-10-15 Online:2012-10-15

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摘要： 目的：探讨N-乙酰半胱氨酸(NAC)对慢性饮酒大鼠肺组织的病理形态及肺组织超氧化物歧化酶(SOD)、丙二醛(MDA)含量的影响。方法：健康雄性大鼠随机分成乙醇组、乙醇+NAC组、对照组各10只，乙醇组和乙醇+NAC组每日给予乙醇液体饲料，乙醇+NAC组给予NAC 300 mg/（kg?d）。8周后处死，观察肺组织病理改变，检测肺组织中的SOD、MDA的含量。结果：乙醇组肺组织可见不同程度的肺泡壁Ⅱ型上皮细胞增生及肺泡间隔炎性细胞浸润，肺泡间隔中胶原纤维沉积增多；乙醇+NAC组胶原纤维沉积和炎性细胞浸润程度比乙醇组轻。乙醇+NAC组肺泡炎、纤维化评分低于乙醇组（P＜0.05或P＜0.01）。乙醇+NAC组肺组织匀浆SOD活力高于乙醇组，MDA含量低于乙醇组（P＜0.05）。结论：NAC提高慢性饮酒大鼠肺组织SOD的活力，降低MDA的含量，减轻肺组织的纤维化程度，对慢性饮酒大鼠肺纤维化有预防作用。

关键词: 乙醇, 肺纤维化, N-乙酰半胱氨酸, 丙二醛, 超氧化物歧化酶

Abstract: Objective: To explore the influence of pulmonary fibrosis induced by chronic ethanol ingestion in rats with N-Acetylcysteine(NAC), through observe the chang of pathogenesis of pulmonary fibrosis with detecting the content of SOD, MDA of lung tissue. Methods: 30 Clean healthy male Sprague-Dawley rats, were randomly divided into alcohol group(10), alcohol+NAC group(10), and control group(10). Feeding quantitative non-ethanol or ethanol Lieber-Decarli liquid diet every day for 8 weeks. Alcohol+NAC group were given NAC 300mg/(kg?d). At study completion, animals were sacrificed, The pathogenesis changes of lung tissue were observed; The activity of SOD and content of MDA of lung tissue were detected. Results: there were varying degree of alveolar and alveolar septal infiltration of inflammatory cells, and there were more deposition of collagen fibers at intervals of alveolar in ethanol group. The similar presents were in ethanol +NAC group, the degree was lower than alcohol group. The degree of alveolitis and the degree of pulmonary fibrosis in alcohol+NAC group were lower than it in alcohol group, (P＜0.01; P＜0.05, respectively). 2. the SOD activity of lung tissue in alcohol+NAC group was higher than alcohol group, the MDA content of lung tissue in alcohol+NAC group was lower than alcohol group (P＜0.05).Conclusion: NAC can raise the SOD activity and decrease the content of MDA of lung tissue in chronic ethanol ingestion rats, restrain the oxidative stress induced by alcohol, decrease the degree of pulmonary fibrosis induced by chronic ethanol ingestion in rats .

Key words: ethanol, pulmonary fibrosis, N-Acetylcysteine, Malondialdehyde, Superoxide dismutase