天津医药

• 实验研究 •    

糖尿病大鼠并发牙周炎血清AGEs浓度变化的研究

曹晓丽1,张云2,李玉民3,李荣华2   

  1. 1. 天津医科大学研究生院；天津市第一中心医院口腔科
    2. 天津市第一中心医院口腔科
    3. 第一中心医院口腔科
  • 收稿日期:2009-11-04 修回日期:2010-03-08 出版日期:2010-06-15 发布日期:2010-06-15
  • 通讯作者: 曹晓丽

Correlative Study of Advanced Glycation End Products ( AGEs) in Diabetic Rats and Periodontitis in Rats

  • Received:2009-11-04 Revised:2010-03-08 Published:2010-06-15 Online:2010-06-15
  • Contact: CAO Xiao-li

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摘要： 摘要 目的：探讨分析糖尿病牙周炎大鼠血清中的糖基化终末产物（AGEs）与牙周炎的相关性。方法：二月龄雄性Wistar大鼠32只随机分成4组每组8只，即正常大鼠对照组（N组）、牙周炎大鼠组（P组）、糖尿病大鼠组(DM组)、糖尿病牙周炎大鼠组(ＤＰ组)。采用一次性腹腔注射链脲佐菌素 (STZ)的方法诱导糖尿病大鼠模型，用正畸结扎丝结扎牙颈部加高糖软食的方法建立牙周炎大鼠模型。二方法结合建立糖尿病牙周炎大鼠模型。各组大鼠的观测指标：体重变化，饮食饮水量，尿量，活动情况，毛色，牙周指标（牙周袋探诊深度和牙龈炎症程度）。实验的第4周和第6周处死大鼠，取下颌骨做病理切片HE染色， ELISA法测血清中的AGEs。结果：4周和6周后，DM组和DP组大鼠出现多食多饮多尿体重相对减轻的症状,血糖大于16.7mmol/l；各组大鼠牙周袋探诊深度值DP组与DM组，DP组与P组，DP组与N组，P组与DM组，P组与N组之间差别均具统计学意义（P<0.05）；血清中AGEs在DP组与N组, DP组与P组，DM组与P组，DM组与N组，比较差别有统计学意义（P<0.05）。糖尿病牙周炎大鼠血清中的AGEs含量明显高于牙周炎组和正常对照组，结论：糖尿病大鼠血中AGEs水平与牙周炎严重程度具有相关性。

关键词: 糖尿病, 牙周炎, 大鼠, 糖基化终末产物, 动物模型

Abstract: Abstract objective：The goal of this study was relevance of AGEs in rat serum and periodontitis severity by detecting AGEs of diabetic periodontitis in the rat analysising the serum content of AGEs .Methods :Two-month-old male wistar rats (n=32)were randomly divided into four groups, the control group (N group), periodontitis group (P group), diabetic group( DM group), diabetic periodontitis group (DP group). Diabetic rats were induced by intraperitoneal injection of streptozotocin ( STZ) . Periodontitis rats were initiated by ligating floss around maxillary molars and High-sugar soft diet . Observation of rats in each group: body weight, food ,water, urine output, activities, periodontal index (periodontal pocket depth, gingival inflammation). Four and six weeks later, animals were sacrificed and the specimens were prepared for histological analysis. serum AGEs were measured in ELISA method. Results: Ligation in rats after four weeks and six weeks, there is more food and more drink urine to reduce the symptoms of the relative weight, blood glucose is greater than 16.7mmol / l.Rats in each group the value of periodontal pocket probing depth comparison between the two groups DP group and DM group, DP group and P group, DP group and N group, P group and DM group, P group and N group the difference were statistical significance (P <0.05).Serum AGEs in the DP group and N group, DP group and P group, DM group and P group, DM group and N group, statistically significant difference (P<0.05). Conclusion : It is relevant that Serum AGEs in diabetic rats and the severity of periodontitis.

Key words: diabetes, periodontitis, rat , AGEs, animal model