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遵义地区汉族人群流行性出血热相关等位基因的分布

秦娜琳,田丹,兰惠琴,孙万邦,张忆雄,罗军敏   

  1. 遵义医学院免疫学教研室
  • 收稿日期:2011-03-03 修回日期:2011-06-24 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 秦娜琳

Research on genetic polymorphisms of HLA- A31, B40, B58, DRB1*16 in the Hans of Zunyi

  • Received:2011-03-03 Revised:2011-06-24 Published:2011-10-15 Online:2011-10-15
  • Contact: Na-Lin QIN

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摘要： 目的:从基因水平调查遵义地区汉族人群与肾综合征出血热相关的HLA-A31、B40、B58、DRB1*16 等位基因频率,并了解其多态性分布状况。方法: 应用聚合酶链反应-序列特异性引物(PCR-SSP) 方法对遵义汉族200名健康个体进行HLA-A、B、DR 位点基因分型，筛选出HLA-A31、B40、B58、DRB1*16阳性标本进一步做高分辨基因亚型分型，进而采用卡方检验对遵义汉族群体的这几组等位基因与其他不同种群的分布情况做比较。结果:遵义地区汉族HLA-A31、B58、DRB1*16 基因各检出一种亚型，HLA-B40共检出4种等位基因亚型，其中B*4001、B*4002、B*4006为常见亚型，B*4063为罕见基因亚型。遵义汉族人群所检出的等位基因亚型基因频率除B*4001和B*4063外均与南方汉族相接近，而与日本人有明显差异。结论:遵义汉族人群HLA-A31、B58、DRB1*16基因亚型相对单一，HLA-B40 基因具有较为丰富的多态性。其分布具有明显的南方汉族人群频率分布特征，又具有自身特点。

关键词: 遵义, HLA-A31、B40、B58、DRB1*16, 基因频率, 多态性, 聚合酶链反应-序列特异性引物

Abstract: Objective :To investigate the frequencies and polymorphism of hemorrhagic fever with renal syndrome (HFRS) associated HLA alleles ,HLA-A31, B40, B58 and DRB1*16 , in the Hans of Zunyi area. Methods: Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to type HLA-A, B, DR genes of 200 unrelated healthy Han individuals in Zunyi area, and then the positive samples of A31, B40, B58 and DRB1*16 were selected and subtyped at high-resolution.. The allelic distribution of these alleles in Zunyi Han populations were compared with other different populations by chi-square test . Results: In HLA-A31, B58, DRB1*16 gene, only one kind of alleles was severally obtained, whereas, in HLA-B40 four kinds of alleles were obtained. Among them, B*4001, B*4002 and B*4006 were common alleles , and B*4063 was one kind of the rare alleles. Comparison of the HLA subtypes frequencies in different populations showed a close relationship of Zunyi Han population with the Han population in Southern China and the distribution of the allele subtypes in Zunyi Han population differed significantly from that of the Japanese. Conclusion: The alleles in HLA-A31、B58、DRB1*16 are relatively single, but the alleles in HLA-B40 have plenty of polymorphism. Their distributions have not only the southern Han’s characteristics but also their own territory feature.

Key words: Zunyi, HLA, Allele frequency, Polymorphism, Polymerase chain reaction-sequence specific primer (PCR-SSP)