天津医药

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慢性饮酒对大鼠肺组织中TIMP-1 表达的影响

王星1,吴琦1,于洪志2,3,杜钟珍4   

  1. 1. 天津市海河医院
    2. 天津市海河医院 天津市呼吸疾病研究所
    3.
    4. 天津海河医院
  • 收稿日期:2011-09-05 修回日期:2012-06-02 出版日期:2012-11-15 发布日期:2012-11-15
  • 通讯作者: 吴琦

The Effect of Chronic Ethanol Ingestion on the expression of

  • Received:2011-09-05 Revised:2012-06-02 Published:2012-11-15 Online:2012-11-15

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摘要： 摘要 目的 通过观察慢性饮酒大鼠的肺组织及基质金属蛋白酶抑制剂-1（TIMP-1）等指标的变化，以探讨酒精与肺纤维化之间的关系及可能的机制。 方法 健康雄性SPF级Sprague-Dawley大鼠20只，随机分成对照组(C组) 10只，每日给定量无酒精液体饲料单笼喂养；酒精组(A组)10只，每日给定量酒精液体饲料单笼喂养，不再提供饮水。16周后，通过Masson染色观察肺间质中胶原沉积的情况，用比色法计算大鼠肺组织中的谷胱甘肽（GSH）和羟脯氨酸（HYP）的含量、通过Elisa方法检测肺组织TIMP-1含量。结果 ⑴Masson染色结果显示：酒精组较对照组在肺泡间隔中胶原纤维沉积增多。⑵与对照组比较，酒精组肺组织GSH含量减少，HYP含量增加，差异均有统计学意义(P<0.05)。⑶与对照组比较，酒精组肺组织TIMP-1表达量升高，差异有统计学意义(P<0.05)。结论 慢性摄入酒精可以引起大鼠肺泡间隔胶原纤维沉积，肺组织中TIMP-1的表达升高。饮酒可能是引起大鼠肺纤维化的原因之一。

关键词: 酒精, 肺纤维化, 基质金属蛋白酶抑制剂-1

Abstract: Abstract Objective: To investigate the relationship between alcohol and Pulmonary fibrosis and possible mechanism by observing the effect of chronic alcohol intake on lung tissues in rats and tissue inhibitor of metalloproteinase-1 glutathione. Methods: Clean healthy male Sprague-Dawley rats 20, weighing 160-200g, were randomly dividedinto control group (C group) 10 and alcohol group (A group) 10, and fed quantitative non-ethanol or ethanol Lieber-DeCarli liquid diet every day in a single cage for 16 weeks. By Masson staining collagen deposition were observed in alveolar interval ; t ere detected.Results: ⑴Masson results show: the alcohol group had more deposition of collagenfibers at intervals of alveolar than the control group.⑵Compared to the group C, the content of GSH in rat lung tissue reduced but HYP increased in group A ,the differences were statistically significant ( P <0.05).⑶TIMP-1 in rat lung tissue of group A were higher than group C, the differences were statistically significant(P <0.05). Conclusion: Chronic alcohol ingestion can cause collagen deposition in alveolar interval and higher expression of TIMP-1 in lung tissue . Alcohol may be one of the reasons for pulmonary fibrosis in rats.

Key words: alcohol, pulmonary fibrosis, tissue inhibitor of metalloproteinase-1 glutathione