天津医药

• 实验研究 •    

SEA体内杀伤S180肉瘤效果及其细胞因子的测定

张云1,孙嘉琳2,王雪2,冯艳敏2   

  1. 1. 天津科技大学
    2.
  • 收稿日期:2009-09-02 修回日期:2009-12-06 出版日期:2010-04-15 发布日期:2010-04-15
  • 通讯作者: 张云

The antitumor effect of SEA in sarcoma 180 and the distribution of the cytokine

  • Received:2009-09-02 Revised:2009-12-06 Published:2010-04-15 Online:2010-04-15
  • Contact: zhang yun

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摘要： 目的：研究SEA抗实验性肿瘤的效果及其对细胞因子IFN-γ的影响，探讨其作用机制。方法：采用S180肉瘤模型进行体内抑瘤实验，分四组，其中SEA高剂量组(n=10)，SEA中剂量组(n=10)，SEA低剂量组(n=10)和对照组(n=10)，前三组每组注射不同浓度的SEA，第四组(n=10)注射生理盐水，接种第5天起测量各组小鼠肿瘤体积，绘制肿瘤生长曲线并计算抑瘤率 。治疗结束后取小鼠血清，从不同水平测定IFN-γ的含量。结果：SEA高剂量组抑瘤率为44.00%，注射高剂量SEA组脾脏指数和组织、血清中IFN-γ的含量明显增加，均与对照组差异显著(*p<0.05)，但肿瘤质量却明显减小(*p<0.05)。结论：SEA通过激活T细胞，分泌细胞因子IFN-γ抑制小鼠体内S180肉瘤生长。

关键词: S180细胞, 金黄色葡萄球菌肠毒素A, ELISA实验, IFN-γ, 抗肿瘤

Abstract: Abstract:Ojective To study the antitumor effect of SEA and the distribution of the cytokine IFN-γ in vivo,and also to investigate the mechanism of killing cancer cells. Methods: Established the tumor xenograft model with S180 cells,and three groups were injected with SEA intraperitoneally (n=10),the fourth group was injected with normal saline intraperitoneally(n=10), measured the tumor volume,drawed growth curve and calculated inhibition ratio, detected the distribution of IFN-γ from different levels.Results:The inhibition ratio of the SEA high-concentration group is 44.00%,and the content of IFN-γ increased both in tissue and in serum(*p<0.05),There is also an obvious difference between the tumor weight and the tumor voume of SEA high-concentration group and the normal saline group. But the tumor weight and the tumor volume of SEA-high concentration group decreased obviously(*p<0.05). Conclusion:SEA has an obvious antitumor effect in S180 tumor, and SEA secrets a mass of cytokine IFN-γ to inhibit sarcoma180 cells.

Key words: sacroma180 cells, SEA, ELISA, IFN-γ, antitumor