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脂多糖与干扰素α联用诱导白血病U937细胞凋亡及其机制

邱元秀,王晓桃,莫东华   

  1. 桂林医学院附属医院
  • 收稿日期:2011-06-08 修回日期:2011-07-19 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 莫东华

  • Received:2011-06-08 Revised:2011-07-19 Published:2012-02-15 Online:2012-02-15

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摘要： 目的：探讨Toll样受体配体内毒素脂多糖（LPS）与干扰素(IFN)-α联用对白血病U937细胞凋亡的作用及其机制。方法：将200 μg/L的LPS和（或）2000 U/mL的IFN-α作用于U937细胞，MTT检测细胞生长抑制率，流式细胞术（FCM）检测细胞凋亡率，半定量逆转录-聚合酶链反应(RT-PCR) 法检测作用48 h后U937细胞中caspase-8 mRNA的表达。结果：LPS与IFN-α联用较单用LPS、IFN-α能明显抑制U937细胞增殖、增加细胞凋亡率（P<0.01），且各药物组较对照组均有统计学意义（P<0.01）。LPS与IFN-α联用作用48h后，caspase-8mRNA的表达水平较IFN-α组、LPS组和对照组升高（P<0.01）。结论：LPS与IFN-α联用能有效抑制白血病细胞的增殖并诱导其凋亡，其机制可能与caspase-8的激活有关。

关键词: 白血病, Toll样受体, U937细胞, 干扰素a

Abstract: Objective：To study the apoptosis of U937 cells induced with lipopolysaccharide (LPS) and interferon-alpha (IFN-a) synergies and its clinical mechanism . Methods：The U937 cells were given with various concentrations of 200ng/ml of LPS and (or) 2000U/ml of IFN-a, the inhibitory ratio was measured by MTT assay, apoptosis rate was detected by flow cytometry ( FCM ) , and the expression of caspase-8 mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR) after48 hours . Results： LPS in combination with IFN-a could inhibit U937 cell proliferation and increase apoptosis rate in comparison with LPS or IFN-a group (P<0.01), and each drug group are statistically significant compared with the control group(P<0.01)。The expression level of caspase-8 mRNA increased significantly 48h after LPS and IFN-a used in comparison with LPS/IFN-a group and the control group(P<0.01)。Conclusion： LPS in combination with IFN-a can inhibit the proliferation and induce apoptosis of U937 leukemia cells,and the apoptosis mechanism may be related with the activation of caspase-8.

Key words: leukemia, toll-like receptors, U937 cells, interferon-alpha