天津医药

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Hhcy及B族维生素干预对大鼠脑血管ICAM-1表达的影响

崔磊,张哲成   

  1. 天津市第三中心医院
  • 收稿日期:2010-11-12 修回日期:2011-04-20 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 张哲成

The research on the expression of ICAM-1 in the brain of rats with hyperhomocysteinemia and after the intervention of B Vitamins

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  • Received:2010-11-12 Revised:2011-04-20 Published:2011-10-15 Online:2011-10-15

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摘要： 目的 探讨高同型半胱氨酸血症（hyperhomocysteinemia，Hhcy）及叶酸、维生素B12和维生素B6干预对大鼠脑皮层血管细胞间粘附分子－1（intercellular adhesion molecule-1，ICAM-1）表达的影响。方法 采用不同饮食结构及干预手段将40只雄性SD大鼠随机分为对照组、Hhcy组、治疗组和预防组，采用高效液相色谱（high performance liquid chromatogram，HPLC）荧光检测技术测定不同时期各组大鼠血浆总同型半胱氨酸（total Homocysteine,tHcy）水平，并于第12周末通过免疫组化法观察ICAM-1在大鼠脑皮层血管的表达情况。结果 第4周末大鼠Hhcy模型制备成功，而第12周末对照组、治疗组和预防组血浆tHcy水平无统计学差异；各组ICAM-1阳性血管个数差异均有统计学意义（P＜0.05），其表达高低顺序依次是Hhcy组（3.23±0.58）＞治疗组（1.54±0.36）＞预防组（0.98±0.23）＞对照组（0.35±0.31）。结论 Hhcy可以促进大鼠皮层脑血管ICAM-1表达，B族维生素可以有效降低血浆tHcy水平，并且对ICAM-1表达有一定的干预作用。

关键词: 高同型半胱氨酸血症, 干预, 细胞间粘附分子－1, B族维生素

Abstract: Objective To observe the expression of ICAM-1 in the brain of rats with Hhcy or after the intervention of folic acid, Vitamins B12 and Vitamins B6. Methods 40 healthy male Sprague-Dawley rats were divided into 4 groups as:normal group, Hhcy group,treat group, prevent group by the way of different foods and different interventions.The plasma total homocysteine (tHcy) levels of rats were assayed by high-performance liquid chromatography method(HPLC) in different time; At the end of 12th week ,the expression of intercellular adhesion molecule-1 (ICAM-1) were detected by immunohistochemical method in the cerebral vessels of rats. Results At the end of 4th week, the Hhcy model were induced successfully; At the end of 12th week ,there were no statistical difference between the treat group,prevent group and normal group; there were significantly statistical differences between the number of masculine cerebral vessels in the 4 groups(P＜0.05), the sequence is Hhcy group（3.23±0.58）＞treat group（1.54±0.36）＞prevent group（0.98±0.23）＞normal group（0.35±0.31）. Conclusions The expression of ICAM-1 were increased in the cerebral vessels of rats with Hhcy.The intervention of B Vitamins could decrease.

Key words: hyperhomocystinemia, intervention, intercellular adhesion molecule-1, B Vitamins