天津医药

• 细胞与分子生物学 • 上一篇    下一篇

自噬相关蛋白在肺鳞癌和腺癌组织中的表达及相关性研究

郭凯,黄波,王晓东   

  1. 辽宁医学院附属第一医院
  • 收稿日期:2013-10-11 修回日期:2014-01-26 出版日期:2014-06-15 发布日期:2014-06-15
  • 通讯作者: 王晓东

Expression and Correlated Analysis of autophagy-related proteins in Human squamous cell carcinoma and adenocarcinoma of lung

  • Received:2013-10-11 Revised:2014-01-26 Published:2014-06-15 Online:2014-06-15

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摘要： 目的：探讨自噬相关蛋白Beclin1、Atg1、mTOR在不同分化程度肺鳞癌(SCC)和腺癌中的表达情况及其相关性。方法：采用免疫组织化学方法，检测82例肺癌组织及17例癌旁正常肺组织Beclin1、Atg1、mTOR的表达情况。结果：癌旁组织中Beclin1、Atg1阳性率高，而mTOR的阳性率较低；SCC和肺腺癌组织中，随分化程度的降低，Beclin1、Atg1呈递减趋势，而mTOR的阳性表达呈递增趋势。相关性分析显示，不同分化程度SCC中Beclin1与Atg1表达呈正相关（R=0.609，P<0.01），Beclin1、Atg1分别与mTOR呈负相关（R=-0.617，-0.444，P<0.01）；不同分化程度肺腺癌中，Beclin1与Atg1表达呈正相关（R=0.458， P<0.01），Beclin1、Atg1分别与mTOR呈负相关（R=-0.497，-0.541，P<0.01）。结论: mTOR随SCC和肺腺癌分化程度的降低，表达逐渐增高，而Beclin1、Atg1表达逐渐降低，提示SCC和肺腺癌的恶变程度与自噬有关。

关键词: 自噬, 肺癌, Beclin1, 自噬相关蛋白1, 雷帕霉素靶蛋白

Abstract: Objective:To explore the expression of Beclin1、autophagy-related protein 1 (Atg1)、target of rapamycin (mTOR) in different squamous cell carcinoma(SCC) and adenocarcinoma of lung and relationship among the three factors. Methods: The expression of Beclin1、Atg1、mTOR in different human SCC and adenocarcinoma of lung were detected by immunohistochemical techniques .There were 17 cases of normal tissue,82 cases of lung cancer. Results: The expression of Beclin1 and Atg1 showed a tendency to increase from high differentiated SCC and adenocarcinoma of lung to low differentiated ones. But mTOR showed a tendency to decrease. Compared with the expression of Beclin1,the expression of Atg1 in SCC and adenocarcinoma of lung presented a positive correlation. And Compared with the one of mTOR,Beclin1 and Atg1 show a negative correlation. Conclusion:Autophagy is related with development and SCC and adenocarcinoma of lung.

Key words: autophagy, lung cancer, Beclin1, autophagy-related protein 1, target of rapamycin