天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (3): 241-244.doi: 10.11958/j.issn.0253-9896.2015.03.005

• 细胞与分子生物学 • 上一篇    下一篇

锌指蛋白185 对胶质瘤细胞增殖影响的研究

陆斌 1， 郑全辉 2△#br# #br#   

  1. 1 河北省唐山市工人医院神经外科 （邮编 063000）； 2 唐山市慢性病临床基础研究重点实验室， 河北联合大学基础医学院
  • 收稿日期:2014-06-06 修回日期:2014-10-27 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: 郑全辉 E-mail:zhqhdlp@sohu.com
  • 作者简介:陆斌 （1974）， 男， 硕士， 副主任医师， 主要从事神经外科学方面研究
  • 基金资助:
    河北省自然科学基金面上项目 （H2013209019）； 唐山市科技局指令性项目 （13130290z）

The effect of zinc finger protein 185 on the proliferation of human glioma cells

LU Bin1, ZHENG Quanhui2△   

  1. 1 Tangshan Gongren Hospital, Tangshan 063000， China； 2 Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Disease; School of Basic Medical Science, Hebei United University

  • Received:2014-06-06 Revised:2014-10-27 Published:2015-03-15 Online:2015-03-15
  • Contact: ZHENG Quanhui E-mail:zhqhdlp@sohu.com

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摘要： 目的 探讨锌指蛋白 ZNF185 在人脑胶质瘤细胞增殖中的作用。方法 标本取自 2011 年 1 月—2013 年12 月于唐山市工人医院就诊， 经病理确诊为胶质瘤患者的肿瘤组织， 以瘤旁组织作对照。提取不同组织总蛋白，Western-blot 检测 ZNF185 的表达。提取瘤旁组织总 RNA， 反转录扩增 ZNF185 编码序列并克隆至 pEGFPC2 质粒，构建 ZNF185 表达载体。采用 Lipofactamine2000 将 ZNF185 表达载体转染人胶质瘤细胞系 SF767， 以转染 pEGFPC2空载体细胞作为对照。采用流式细胞仪检测细胞周期变化； MTT 法检测细胞增殖活性。结果 与瘤旁组织相比，ZNF185 在人脑胶质瘤组织中表达显著降低（P < 0.01）； 转染 ZNF185 表达载体的胶质瘤细胞与对照细胞比ZNF185 的表达显著增加（P < 0.01）； 过表达 ZNF185 导致 SF767 细胞 G0~G1 期细胞比例显著增加（P < 0.05）， 而 S期细胞比例减少（P < 0.05）。同时， 过表达 ZNF185 也导致 SF767 细胞的增殖速度显著降低（P < 0.05）。结论 ZNF185 在人脑胶质瘤细胞中发挥抑制细胞增殖的作用。

关键词: 神经胶质瘤, 细胞周期, 细胞增殖, 锌指, 转染, ZNF185

Abstract: Objective To explore the role of zinc finger protein (ZNF)185 in the proliferation of human glioma cells. Methods Human glioma tissues and tumor adjacent tissues were obtained from glioma patients diagnosed pathologically in Tangshan Gongren Hospital from January 2011 to December 2013. Total protein was extracted from different tissues. The ZNF185 expression was detected by Western-blot assay. Total RNA was extracted from tumor adjacent tissues. ZNF185 coding sequence was obtained by RT-PCR and inserted into pEGFPC2 plasmid to construct the ZNF185 expression vector. Lipofactamine2000 was used to transfect the ZNF185 expression vector to human glioma cell SF767. pEGFPC2 blank vector transfected SF767 cells were used as control. Changes of cell cycle were analyzed by flow cytometry, and cell proliferation was analyzed by MTT assay. Results The expression of ZNF185 decreased significantly in human glioma tissues compared
to that of tumor adjacent tissues（P < 0.01). The over expression of ZNF185 in SF767 resulted in the increased proportion of cell cycle G0-G1phase, but decreased proportion of S phase（P < 0.05). Furthermore, the cell proliferation was significantly inhibited in the ZNF185 over-expressed SF767 cells compared with that of blank vector transfected cells（P < 0.05). Conclusion ZNF185 plays an inhibitory role in cell proliferation of human brain glioma cells.

Key words: glioma, cell cycle, cell proliferation, zinc fingers, transfection, ZNF185