天津医药

• 论著 •    

MicroRNA-93在骨肉瘤组织中的表达及其意义

何敏1,刘水红2,杨志强3,高志增1   

  1. 1. 南昌大学第一附属医院
    2. 萍乡市第一人民医院
    3. 南昌大学
  • 收稿日期:2011-12-27 修回日期:2012-06-27 出版日期:2012-12-15 发布日期:2012-12-15
  • 通讯作者: 高志增

Expression of MicroRNA-93 in Osteosarcoma and the Significance

  • Received:2011-12-27 Revised:2012-06-27 Published:2012-12-15 Online:2012-12-15

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摘要： 目的：分析MicroRNA-93（miR-93）在骨肉瘤组织中的表达，探讨其对骨肉瘤细胞的增殖及细胞周期的意义。方法：采用TagMan MGB探针法定量分析38例骨肉瘤组织及对应软骨组织中miR-93的表达；构建miR-93过表达载体，并合成miR-93的反义核苷酸序列，分别转染骨肉瘤细胞U2-OS和MG-63。采用MTT比色法检测骨肉瘤细胞增殖的改变情况，利用流式细胞仪检测骨肉瘤细胞周期的变化情况。结果：在38例骨肉瘤标本中，65.8%（25/38）的骨肉瘤组织miR-93表达明显高于对应软骨组织（P<0.05）。miR-93过表达载体可以促进骨肉瘤细胞的生长，使细胞周期G0/G1期明显降低，而S期比例明显增加；反义miR-93转染骨肉瘤细胞U2-OS和MG-63后，miR-93的表达明显降低，U2-OS和MG-63骨肉瘤细胞生长受到明显抑制，其生长主要停滞在 G0/G1期，而 S期和 G2/M期细胞的比例下降。结论：miR-93在骨肉瘤组织中表达明显上调，其可以通过调节细胞周期中G1/S期的转换而影响骨肉瘤细胞是生长，可能为骨肉瘤基因表达调控研究提供新的靶基因。

关键词: 骨肉瘤, 微RNAs, 细胞增殖, 细胞周期, 转染, microRNA-93

Abstract: Objective: To analyse the expression of microRNA-93 (miR-93) in osteosarcoma and investigate the effect of miR-93 on the proliferation and cell cycle in osteosarcoma cell lines. Methods: To detect the expression of miR-93 in 38 pairs of osteosarcoma and cartilaginous tissues using TagMan MGB Real-time PCR. To construct the miR-93 overexpressed vector and synthesis the miR-93 antisense nucleotide sequence. Then they were transfected into osteosarcoma cell lines U2-OS and MG-63. Cell proliferation was detected by MTT assay and cell cycle was detected by flow cytometry. Results: 65.8%（25/38）of miR-93 was overexpressed in osteosarcoma compared to cartilaginous tissue (P<0.05). Overexpression of miR-93 could promote the growth of osteosarcoma cell lines and delay the cell cycle in S phase. The reduction of miR-93 could inhibit the growth of osteosarcoma cell lines and delay the cell cycle in G0/G1 phase. Conclusions: miR-93 was overexpressed in osteosarcoma and miR-93 could regulate the growth of osteosarcoma cells via mediating the transition of G1/S phase, which may become a targeted gene of osteosarcoma.

Key words: osteosarcoma, microRNAs, cell proliferation, cell cycle, transfection, miR-93