天津医药

• 实验研究 •    

水蛭素对动脉内皮保护作用的实验研究

梁桂文,姜敏辉   

  1. 南通大学附属医院
  • 收稿日期:2011-10-24 修回日期:2012-07-06 出版日期:2012-12-15 发布日期:2012-12-15
  • 通讯作者: 姜敏辉

Study on the Protective Effects of hirudo to arterial endothelium

Gui-Wen LIANG1,   

  • Received:2011-10-24 Revised:2012-07-06 Published:2012-12-15 Online:2012-12-15

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摘要： 目的：探讨水蛭在保护动脉粥样硬化动脉内皮的作用及可能机制。方法：55只大耳白兔随机分为正常对照组（A组）予普通喂养，模型组予高脂喂养9周。确定模型成功后模型组再次分为未处理组（B组）继续高脂喂养；低剂量水蛭组（C组）加用水蛭粉0.5 g/（kg?d）；高剂量水蛭组（D组）加用水蛭粉1.5 g/（kg?d）；阿托伐他汀组（E组）加用阿托伐他汀2.5 mg/（kg?d）。治疗8周后比较各组的胸主动脉大体形态、组织形态差异和血清总胆固醇（TC）、低密度脂蛋白（LDL）血脂、一氧化氮（NO）、内皮素（ET）-1、前列环素（PG）F-1-α、血栓烷（TX）B2及C-反应蛋白（CRP）等指标差异。结果：B、C、D、E组平均斑块面积分别为74%、45% 、21%、20%。D组TC、TXB2、ET-1、CRP低于B组，NO、PGF-1-α显著高于B组，TC、LDL、NO高于E组，ET-1低于E组，差异有统计学意义(均P<0.05)。结论：水蛭可能通过升高NO、PG F-1-α、抑制TXB2、ET-1及抑制炎症反应来保护动脉内皮。

关键词: 动脉粥样硬化, 水蛭素, 胆固醇, 胆固醇, LDL, 一氧化氮, 血栓烷B2, 疾病模型

Abstract: Objective: atherosclerosis model in rabbits were established by feeding high-fat diets. to observed the effects on protect the vascular endothelium of hirudo and atorvastatin. Methods: feeding group A (normal diets) and model group (high-fat diets) 9 weeks, after the successful establishment of models divided the alive rabbits into four groups: rabbits in group B were fed with high-fat diets; rabbits in group C were fed with high-fat diets which mixed hirudo medicinalis powder 0.5 g/Kg/d; rabbits in group D were fed with high-fat diets which mixed hirudo medicinalis powder 1.5g/Kg/d; rabbits in group E were fed with high-fat diets which mixed atorvastatin 2.5mg/Kg/d. At the end of 17th week, blood samples were taken and made into serum for testing assay lipid , NO, ET-1, PGF-1-α, TXB2, and CRP. Then all rabbits were sacrificed, and the thoracic aortas of them were taken out. The arteries were embedded in paraffin and cut into transverse sections. They were subjected to the HE-staining which could observe changes of the arteries in morphological. Results: Macroscopic observation：there was no difference of the plaque area between group D and group E, and both smaller than group B(p＜0.05). The TC, LDL, TXB2, ET-1 and CRP of group D higher than group B, while the NO and PGF-1-α lower than group B. The TC, LDL and NO of group D higher than group B while the ET-1 lower than group B. All the differences had statistical significance. Discussion: Hirudo has a remarkable effect on protect the vascular endothelium . Its mechanism is related to increasing NO and PGI2, while decreasing TXB2 and ET-1.

Key words: atherosclerosis HIRUDINcholesterol cholesterol, LDL Nitric Oxidethromboxane B2disease models, animalatorvastatin, 疾病模型