天津医药

• 实验研究 •    

灵芪蠲肝液抗CCl4诱导的肝损伤机制的研究

盛云建1,吴刚1,陈炘2,邓存良1   

  1. 1. 泸州医学院附属医院
    2. 四川省内江市第一人民医院
  • 收稿日期:2011-06-08 修回日期:2011-09-18 出版日期:2012-03-15 发布日期:2012-03-15
  • 通讯作者: 邓存良

The Effects of LingQiJuanGan Liquor on Toll-like Receptor 4 in Rats with CCl4-induced Chronic Liver Injury

  • Received:2011-06-08 Revised:2011-09-18 Published:2012-03-15 Online:2012-03-15

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摘要： 摘要 目的 研究灵芪蠲肝液抗四氯化碳诱导的肝损伤作用及其机制。 方法 40％的四氯化碳复制大鼠肝损伤模型，造模第4周起予灵芪蠲肝液，于0、4、6、8、10周检测Kupffer细胞(KCs)Toll样受体4 mRNA （TLR4mRNA）、肝组织TLR4和核转录因子κB（NF-κB）蛋白表达及血清肿瘤坏死因子α（TNF-α）、白介素1β（IL-1β）和内毒素水平。结果 与模型组相比，灵芪蠲肝液治疗组能明显降低血清TNF-α、IL-1β和内毒素水平（P <0.001），且KCs TLR4mRNA、肝组织TLR4和NF-KB蛋白表达也明显降低（P <0.001）。血清内毒素水平与KCs TLR4mRNA表达成正相关(r=0.845, P<0.001)。 结论 灵芪蠲肝液可通过降低血清内毒素、肝组织TLR4蛋白表达及信号转导，减少炎症因子生成起抗大鼠实验性肝损伤的作用。

关键词: 灵芪蠲肝液, 慢性肝损伤, 肿瘤坏死因子α, Toll样受体4, 大鼠

Abstract: Abstract Objective To dynamic observe the protective effects and mechanism of LingQiJuanGan(LQL) in rats with CCl4-induced chronic liver injury. Methods The chronic liver injury in rats was induced by 40% CCl4, the therapic group was given LQL by intragastric administration from the fourth week. Blood and liver were collected at the same time at the end of the 0, 4th, the 6th, the 8th and the 10th week. The level of serum endotoxin , tumor necrosis factor-α (TNF-α) and interleukin 1β( IL-1β) were detected. The expression of nuclear factor kappa B (NF-κB) and toll-like receptor 4(TLR4) protein in hepatic tissue were assayed by immunohistochemistry. The expressions of TLR4 mRNA in Kupffer cells(KCs) were measured by reverse transcriptase polymerase chain reaction (RT-PCR). Results Compared with the model group, LQL therapic group noticeable decreased the level of serum endotoxin , TNF-α and IL-1β（P <0.001）. LQL therapic group had lower expression of the TLR4 mRNA in KCs and NF-κB and TLR4 protein in hepatic tissue than that of model group（P <0.001）. A positive correlation was found between serum endotoxin levels and the expression of TLR4 mRNA in KCs（r=0.845, P<0.001） Conclusion The inhibition of expressions of TLR4 and endtoxin in chronic liver injury induced by CCl4 in rats may account for the anti liver inflammation effects of LQL.

Key words: LingQiJuanGan Liquor, chronic liver injury, TNF-α, TLR4, Rat