天津医药

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骨髓基质细胞和神经干细胞体外共培养的实验研究

林欣   

  1. 天津市环湖医院
  • 收稿日期:2010-08-18 修回日期:2010-12-02 出版日期:2011-03-15 发布日期:2011-03-15
  • 通讯作者: 林欣

The experimental study of MSCs co-culture with NSCs in vitro

xin lin   

  • Received:2010-08-18 Revised:2010-12-02 Published:2011-03-15 Online:2011-03-15
  • Contact: xin lin

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摘要： 目的 研究神经干细胞（neural stem cell, NSC）和骨髓基质细胞(bone marrow stromal cell，BMSC)两种细胞一起培养时，两种细胞的增殖与分化的关系。方法 将标记BrdU的NSCs和MSCs按接种密度分为6种比例，每种比例再分为增殖和分化两组，各按培养时间分别接种4块（3、7、14、28天）。增殖组进行BrdU免疫细胞化学染色，分化组分别进行BrdU和NSE、GFAP、MAP2、GalC免疫细胞化学双染色。 结果 共培养各组神经干细胞分化为神经元的比例增高，随着NSCs比例的提高，分化的神经元和星形胶质细胞越多。共培养组部分BMSC可以转化为神经元样细胞和星形胶质细胞。 结论 骨髓基质细胞可以促进神经干细胞分化为神经元，而且在神经干细胞诱导下部分BMSC可以转化为神经元样细胞。

关键词: 骨髓基质细胞, 神经干细胞, 培养, 体外, 大鼠

Abstract: Objective： to study the proliferation and differentiation of neural stem cell (NSC) and bone marrow stromal cells (BMSC) when two cells are co-culture. Methods： MSCs and NSCs labeling with BrdU markers are divided into six groups by their proportion, each group is divided into the proliferation and differentiation groups，each group are divided into 4 groups(3 and 7, 14 and 28 days). Proliferation group is stained by BrdU immunocytochemical staining, differentiation groups are stained by BrdU and GFAP，NSE，MAP2,GalC double immunocytochemical staining. Result: The bone marrow stromal cells can improve the differentiation of the neural stem cells. The proportion of each neuron and astrocytes can increase by more NSC. BMSC can be induced their differentiation into neuron-like and astrocytes by NSCs, Conclusion: Bone marrow stromal cells can induce the neural stem cells into neurons and part of them can be induced their differentiation into neuron-like and astrocytes by NSCs.

Key words: neural stem cell, bone marrow stromal cell, culture, vitro, rat