天津医药

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狗食管下括约肌处射频控温热凝术后病理学及神经递质变化的研究

周晓丹1,王洪涛1,刘义宾2,汪忠镐3   

  1. 1. 郑州大学第四附属医院消化内科
    2. 河南省郑州市南阳路169号附10号郑州大学第四附属医院神经外科
    3. 第二炮兵总医院胃食管反流病中心
  • 收稿日期:2013-10-22 修回日期:2013-12-28 出版日期:2014-07-15 发布日期:2014-07-15
  • 通讯作者: 王洪涛

Study on Pathology and Neurotransmitters of Esophageal Sphincter after Radiofrequency Thermocoagulation in Dogs

  • Received:2013-10-22 Revised:2013-12-28 Published:2014-07-15 Online:2014-07-15

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摘要：

【摘要】目的探讨射频控温热凝术（RFT）对家狗食管下括约肌（LES）处病理组织及神经递质胆碱乙酰转移酶（ChAT）、血管活性肠肽（VIP）和一氧化氮合酶（NOS）表达的影响及可能机制。方法15只家狗随机分为3组：进行胃镜检查并饲养3个月（Sham）组、RFT后饲养24h（RFT+24h）组、RFT后饲养3个月（RFT+3m）组。取LES组织采用 HE染色法观察3组的病理组织学变化，免疫组化法观察Sham组和RFT+3m组ChAT、VIP和NOS的表达情况。结果 HE染色结果显示Sham组接近正常组织，RFT+24h组黏膜出现急性炎症反应和结构破坏，RFT+3m组黏膜出现慢性炎性反应和结构重构；免疫组化结果显示RFT+3m组与Sham组相比，ChAT明显升高，VIP、NOS明显下降（均P< 0.01）。结论RFT可使狗的LES厚度和压力增加，并造成局部组织内神经递质发生表达变化。

关键词: 胃食管反流, 食管括约肌, 下段, 胆碱O-乙酰转移酶, 血管活性肠肽, 一氧化氮合酶, 狗, 射频控温热凝术

Abstract: abstract:Objective To investigate the pathological and the expression of choline acetyltransferase(ChAT),vasoactive intestinal peptide(VIP),nitric oxide synthase(NOS) at lower esophageal sphincter(LES) against family dogs,which were operated on radiofrequency thermocoagulation(RFT).Methods A total of 15 dogs were randomly divided into three groups,Sham group:only underwent gastroscopy and feeding 3 months(n=5);RFT+24h group:feeding 24 hours after RFT(n=5);RFT+3m group:feeding 3 months after RFT(n=5). Observed the pathological of LES at 3 groups after HE staining,and the expression of ChAT, VIP and NOS by immunohistochemical method between Sham group and RFT+3m group.Results Sham group showed close to the normal tissue,RFT+24h group showed active inflammatory reaction and structural damage,RFT+3m group showed chronic inflammatory reaction and remodeling;Immunohistochemistry showed RFT+3m group compare with Sham group,ChAT were significantly increased,VIP,NOS were significantly decreased(all P<0.01).Conclusion After dogs operated by RFT,the thickness and pressure of LES were increasing，and expression of neurotransmitters in local tissue was changing.

Key words: gastroesophageal reflux, esophageal sphincter, lower, choline o-acetyltransferase, vasoactive intestinal peptide, nitric oxide synthase, dogs, 射频控温热凝术