天津医药

• 实验研究 •    

脑脊液引流影响颅脑创伤后免疫操作的影响

张洪伟1,闫华2,武俏丽3,张国斌2,刘暌4,只达石5   

  1. 1. 天津医科大学第二医院
    2. 天津市环湖医院
    3. 环湖医院神经外科研究所
    4. 天津市人民医院神经外科
    5. 天津市环湖医院 亚低温治疗中心
  • 收稿日期:2011-05-03 修回日期:2011-08-24 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 闫华

A study on changes of antibrain-antibody and interleukin-1βin rabbits with traumatic brain injury after drainage of cerebrospinal fluid

  • Received:2011-05-03 Revised:2011-08-24 Published:2012-01-15 Online:2012-01-15

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摘要： 目的 研究脑脊液引流后创伤大白兔血清中抗脑抗体和IL-1β水平的变化趋势，探讨脑脊液引流对颅脑创伤后免疫损伤的治疗作用。方法 32只大白兔随机分为对照组和脑脊液引流组，每组16只。脑损伤后0-7天取患兔脑脊液1ml，对照组将脑脊液回注入脑池；脑脊液引流组弃去脑脊液。收集第1、3、7、14、21天血清，以ELISA法检测白介素-1β(IL-1β)和抗脑抗体(ABAb)的浓度，并进行对比。结果 脑脊液引流组IL-1β和ABAb浓度较对照组降低（P<0.05）。伤后21天，脑脊液引流组死亡率和致残率较对照组降低，而痊愈率升高。结论 脑脊液引流可以减少脑组织周围的炎性因子，可以减轻颅脑创伤后的免疫损伤。

关键词: 颅脑创伤, 脑脊液引流, 免疫, 抗脑抗体

Abstract: Objective To study changes of antibrain-antibody (ABAb)and interleukin-1β in rabbits with traumatic brain injury with drainage of cerebrospinal fluid therapy and to explore the protective effect of this therapy in rabbit—TBI—models established by lateral fluid percussion machine. Methods 32 rabbits were divided into four groups randomly (16 in control group, 16 in CSF drained group). Rabbits in control group were drained CSF and injected into cistern; rabbits in CSF drained group were drained CSF only. 2 groups rabbits were drained CSF 1ml everyday until a week and phlebotomized and tested with ELISA to observe the changes of IL-1βand ABAb at the time points 1 d, 3 d, 7d, 14d, 21d. Results Levels of IL-1βand ABAb in CSF drained group were lower than that in control group(P<0.05). The mortality rate and disability rate of CSF drained group were lower than those of control group, while the cure rate was the higher than that of control group. Conclusion Drainage of cerebrospinal fluid can help to decrease levels of antibrain-antibody and interleukin-1β. Drainage of cerebrospinal fluid therapy has protective effect on immunologic injury after traumatic brain injury.

Key words: Craniocerebral trauma, cerebrospinal fluid, immune, Antibrain antibody