关键词: 人乳头瘤病毒16, 感染, 宫颈肿瘤, 问卷调查, 危险因素, 焦磷酸测序技术

Abstract: Abstract: Objective To investigate the incidence and risk factors of human papillomavirus type 16 (HPV16) infec⁃ tion among women through health screenings in Tianjin urban area. Methods A total of 2 000 women from Tianjin urban area were recruited for this study, and potential risk factors were investigated by questionnaire as well as by collecting cervi⁃ cal exfoliated cells from the participants. HPV type was examined using polymerase chain reaction and pyrosequencing tech⁃ nology, then the risk factors of HPV16 infection were examined by chi square test and the multiple factor logistic regression analysis. Results Among those 2 000 women who received health screenings, a total of 271 cases were HPV positive (13.55%). HPV16 accounts for the most common human papillomavirus types (39.5%, 107/271) while HPV58 account for 15.13%（41/271）and HPV18 account for 9.59% (26/271). Factors related to HPV16 infection were investigated include smoking history, initial age of sex (>25 years old or not) , number of pregnancies (≥2 or not), number of sexual partners (≥2 or not) and contraception measures (condom or other ways) with χ2=6.268，8.540，4.951，7.123，6.053，5.019, respectively. (P < 0.05). Age, alcohol intake, education level and previous gynecologial examination did not differ significantly among HPV16 positive patients compared to those with negative HPV. Multiple factor Logistic regression model analysis showed that the independent factors were smoking history and initial age for sex（≤25 years old）(P < 0.05). Conclusion HPV16 is the most common type of cervical infection among women in Tianjin urban area. Changing inappropriate sexual behavior and smoking cessation are effective measures to prevent HPV16 infection and reduce incidence of cervical cancer.

Key words: human papillomavirus 16, infection, uterine cervical neoplasms, questionnaires, risk factors, pyrosequencing