天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (8): 942-945.doi: 10.11958/j.issn.0253-9896.2015.08.031

• 综述 • 上一篇    下一篇

代谢组学技术在临床诊断中的研究进展

李彦东，吴琪   

  1. 东北林业大学医院检验科
  • 收稿日期:2014-11-10 修回日期:2015-03-19 出版日期:2015-08-15 发布日期:2015-08-15
  • 通讯作者: 吴琪 E-mail:liyandong2014@sina.com
  • 作者简介:李彦东（1975），男，硕士，主管检测师，主要从事生物工程研究

Current research on metabonomics detection in clinical diagnosis

LI Yandong, WU Qi   

  1. Department of Clinical Laboratory, Hospital of Northeast Forestry University, Harbin 150040, China
  • Received:2014-11-10 Revised:2015-03-19 Published:2015-08-15 Online:2015-08-15

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摘要： 代谢组学是系统生物学的重要组成部分。研究显示，代谢组学在临床诊断过程中有着不可比拟的作用。当机体受到外源性或内源性刺激时，体内小分子代谢物的种类及含量会发生显著改变，代谢组学技术通过对体内复杂代谢物的动态变化进行分析，分析代谢物变化与机体病理或生理变化的直接相关性。随着分析技术的不断发展，核磁共振、色谱-质谱联用等先进技术被广泛应用于代谢组学的研究中。目前临床运用代谢组学技术可以全面分析患者体液中代谢物的动态变化，发现体液中与疾病密切相关的特征性生物标志物，从而实现疾病的早期临床诊断。本文主要对代谢组学技术及其在临床诊断过程中的广泛应用作一综述。

关键词: 代谢组学, 临床诊断, 生物标志物, 代谢产物, 核磁共振, 综述

Abstract: Metabonomics is an important part of systems biology. Many research shows that metabonomics has an poten⁃ tial incomparable advantages in clinical diagnosis. When human body was stimulated by exogenous or endogenous factor, it could respond throughalteration of low molecular weight metabolites. Therefore, researches of the dynamic changes of com⁃ plex metabolites could explore the pathological or physiological changes in the body. With advance of modern technologies nuclear magnetic resonance and chromatography couple to mass spectrometer are widely applied to the metabonomics. Now, comprehensive analysis the dynamic changes of patients in vivo then setting up relationship between biomarkers and related diseases become possible through the application of Metabolomics in clinical. Finally, we discuss the realization of disease diagnosis by metabolome analysis.

Key words: metabonomics, clinical diagnosis, biomarkers, metabolite, NMR, review