天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (11): 1288-1291.doi: 10.11958/j.issn.0253-9896.2015.11.018

• 实验研究 • 上一篇    下一篇

枸杞多糖通过诱导巨噬细胞极化抗胰腺癌的研究

杨青 1， 白光 2△， 王巍 2， 包翠芬 1， 翟振华 2   

  1. 1辽宁锦州， 辽宁医学院人体解剖与组织胚胎学教研室 （邮编121000）； 2辽宁医学院附属第一医院
  • 收稿日期:2015-03-17 修回日期:2015-07-06 出版日期:2015-11-15 发布日期:2015-11-15
  • 通讯作者: 白光 E-mail： blueskydavid@163.com E-mail:734562938@qq.com
  • 作者简介:杨青 （1988）， 男， 硕士研究生在读， 住院医师， 主要从事肝胆外科肿瘤方面的研究
  • 基金资助:
    吴阶平医学基金项目 （320.6750.1281）； 辽宁省工业企业科技特派员行动项目

Anti-tumor effects of lycium barbarum polysaccharide on pancreatic cancer cells by polarization of macrophages#br#

YANG Qing1, BAI Guang2△, WANG Wei2, BAO Cuifen1, ZHAI Zhenhua2   

  1. 1 Department of Human Anatomy & Histology and Embryology, Liaoning Medical University, Jinzhou 121000, China; 2 The First Affiliated Hospital, Liaoning Medical College
  • Received:2015-03-17 Revised:2015-07-06 Published:2015-11-15 Online:2015-11-15
  • Contact: BAI Guang E-mail： blueskydavid@163.com E-mail:734562938@qq.com

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摘要： 目的 探讨枸杞多糖 （LBP） 通过诱导巨噬细胞极化成一型巨噬细胞 （M1） 抗小鼠胰腺癌细胞 LTPA 的功效。方法 构建小鼠 CB-17SCID 皮下 LTPA 成瘤模型， 随机分为荷瘤模型组 （10 只） 及 LBP 治疗组 （10 只）， LBP 治疗组每日 10 mg/kg LBP 灌胃， 荷瘤模型组每日同等剂量的生理盐水灌胃。将含有相同数量的小鼠巨噬细胞 Raw264.7 随机分为不同 LBP 浓度的实验组和对照组， MTT 法检测各实验组与对照组中 Raw264.7 的光密度 （OD） 值； ELISA 法检测 LBP 质量浓度为 100 mg/L 的实验组与对照组中 Raw264.7 分泌白细胞介素 （IL） -12 及 IL-10 的水平； 流式细胞仪检测 LBP 质量浓度为 100 mg/L 的实验组与对照组中 Raw264.7 表面蛋白 CD16/32 及 CD206 的水平。小鼠荷瘤 3 周后剖瘤称质量并计算瘤体体积， 检测 LBP 对皮下肿瘤生长的影响， HE 染色和 KI-67 免疫组化法检测 LBP 对瘤组织镜下的改变及对 LTPA 增殖的影响。结果 100 mg/L LBP 对 Raw264.7 的生长有明显促进作用（P < 0.01）， 并使Raw264.7 高表达 CD16/32， 低表达 CD206； 高分泌 IL-12、 低分泌 IL-10。LBP 治疗组瘤块质量、 体积及 KI-67 的表达量显著低于荷瘤模型组 （P < 0.01）， 镜下肿瘤坏死区范围明显大于荷瘤模型组。结论 LBP 能够通过诱导巨噬细胞极化成 M1 状态达到抗 LTPA 的作用。

关键词: 巨噬细胞, 胰腺肿瘤, 枸杞多糖, 巨噬细胞极化, 抗胰腺癌, 1 型巨噬细胞, 2 型巨噬细胞

Abstract: Objective To explore the effects of lycium barbarum polysaccharide (LBP) on restraining the mouse pancreatic cancer cells LTPA by the polarization of macrophages to type 1 macrophages (M1). Methods LTPA tumor model of the subcutaneous CB-17SCID mice was constructed. Model mice were randomly divided into tumorbearing model group (n=10) and LBP treatment group (n=10). The LBP treatment group was fed 10mg/kg LBP every day, and the tumor-bearing model
group was fed the same dose of normal saline. The same amount of macrophages Raw264.7 was randomly divided into the control group and experimental groups (different concentrations of LBP). MTT assay was used to detect the optical density (OD) of Raw264.7 in experimental groups and control group. ELISA was used to detect the levels of the interleukin (IL)-12 and IL-10 in experimental group (LBP was 100 mg/L) and the control group. Flow cytometry was used to test the levels of the
membrane protein CD16/32 and CD206 in experimental group (LBP was 100 mg/L) and the control group. The tumor mass was weighted and the volume was calculated after three weeks. The effects of LBP on the growth of subcutaneous tumor were detected. HE staining and KI-67 staining were used to detect the microscopic changes of tumor and the proliferation of the LTPA. Results The dose of 100 mg/L LBP can promote the growth of the macrophages Raw264.7 (P < 0.01), and induced the high expression of CD16/32 and low expression of CD206, high secretion of IL- 12 and low secretion of IL- 10. The weight, volume of the tumor and the expression of KI-67 were significantly lower in experimental group than those in the control group (P < 0.01). The microscopic necrosis area range of tumor was larger than that of control group. Conclusion The LBP has the effect of restraining LTPA by the polarization of macrophages to M1.

Key words: macrophages, pancreatic neoplasms, lycium barbarum polysaccharide, the polarization of macrophages, anti-tumor about pancreatic cells, type 1 macrophages, type 2 macrophages