天津医药 ›› 2016, Vol. 44 ›› Issue (2): 166-169.doi: 10.11958/20150151

• 实验研究 • 上一篇    下一篇

慢性铅暴露对大鼠脑组织 XRCC1 mRNA 表达的影响及其与氧化应激的关系

李炜娟 1,2, 任清风 1,3, 徐群英 1, 张中伟 1, 李伟 1, 冯建高 1, 任晓慧 1, 肖元梅 1?   

  1. 1南昌大学公共卫生学院 (邮编330006); 2南昌大学抚州医学院; 3九江学院临床医学院·附属医院
  • 收稿日期:2015-09-07 修回日期:2015-11-02 出版日期:2016-02-15 发布日期:2016-02-15
  • 通讯作者: ∆通讯作者 E-mail: xym72@163.com E-mail:877761070@qq.com
  • 基金资助:
    国家自然科学基金资助项目(81160342); 江西省自然科学基金资助项目(20122BAB205047); 江西省教育厅科技项目 (GJJ11312)

Effects of chronic lead exposure on expression of XRCC1 gene mRNA and its relationships with oxidative stress in brain tissues of rats

LI Weijuan1,2, REN Qingfeng1,3, XU Qunying1, ZHANG Zhongwei1, LI Wei1, FENG Jiangao1, REN Xiaohui1, XIAO Yuanmei1?   

  1. 1 School of Public Health, Nanchang University, Nanchang 330006, China; 2 Fuzhou Medical College of Nanchang University; 3 Jiujiang University Clinical Medical College, Jiujiang University Hospital
  • Received:2015-09-07 Revised:2015-11-02 Published:2016-02-15 Online:2016-02-15
  • Contact: ∆Corresponding Author E-mail: xym72@163.com E-mail:877761070@qq.com

摘要: 目的 观察饮水铅暴露对大鼠大脑皮质、 小脑、 海马组织中 X 线交错互补修复基因 1(XRCC1)mRNA 表达的影响及其与氧化应激的关系。方法 40 只 SD 大鼠根据体质量按随机区组法分对照组和 4 个铅暴露组: 最低剂量组、 低剂量组、 中剂量组、 高剂量组, 对照组自由饮用去离子水, 4 个铅暴露组分别饮用 100、 200、 400、 800 mg/L 的醋酸铅溶液, 连续染毒 60 d 后取大脑皮质、 小脑和海马。RT-PCR 技术检测脑组织 XRCC1 mRNA 的表达量, 并测定脑组织铅、 过氧化氢酶 (CAT)、 谷胱甘肽 (GSH) 和过氧化氢 (H2O2) 的含量。结果 与对照组比较, 铅暴露组大鼠大脑皮质、 小脑和海马中 XRCC1 mRNA 表达量、 脑铅的含量和 H2O2水平均升高(P<0.05); 而 CAT、 GSH 含量基本低于对照组 (P < 0.05); 相关性分析显示铅暴露组大鼠大脑皮质、 小脑和海马中 XRCC1 mRNA 表达量与脑组织铅含量呈正相关(r 分别为 0.608、 0.438、 0.470, P<0.01), 与 CAT、 GSH 呈负相关 (r 分别为-0.343、 -0.465、 -0.423, -0.383、 -0.489、 -0.366, P<0.05), 与 H2O2 呈正相关(r 分别为 0.455、 0.517、 0.342, P<0.05)。结论 铅可通过诱导细胞氧化应激而影响 XRCC1 mRNA 的表达。

关键词: 铅, X 线交错互补修复基因 1, 氧化应激, 过氧化氢酶, 谷胱甘肽, 过氧化氢, 脑组织

Abstract: Objective To observe the effects of lead exposure through drinking water on the expression of XRCC1 mRNA in cerebral cortex, cerebellum and hippocampus of rats and its relationship with oxidative stress. Methods Forty SD rats were divided randomly into five groups: control group and four exposure groups (100 mg/L, 200 mg/L, 400 mg/L and 800 mg/L lead acetate for 60 days respectively). The expression of XRCC1 mRNA in brain was detected by RT-PCR technique after separation of cerebral cortex, cerebellum, and hippocampus. At the same time, lead content in brain tissue and catalase (CAT), glutathione (GSH) and hydrogen peroxide (H2O2) were also detected. Results Compared with control group, the expression of XRCC1 mRNA, content of lead and H2O2 levels were significantly higher in cerebral cortex, cerebellum and hippocampus of exposure groups (P < 0.05), whereas the contents of CAT and GSH were significantly lower (P < 0.05). There was a positive correlation between lead level and the expression of XRCC1 mRNA in cerebral cortex, cerebellum and hippocampus of exposure groups (r=0.608, 0.438 and 0.470, P<0.01). There was a negative correlation between the lead level and CAT and GSH (r=-0.343, -0.465、 -0.423, -0.383, -0.489 and -0.366, P<0.05). A positive relationship was found between the lead level and H2O2 (r=0.455, 0.517 and 0.342, P<0.05). Conclusion Lead exposure can affect the expression of mRNA gene in XRCC1 through inducing cell oxidative stress.

Key words: lead, XRCC1, oxidative stress, catalase, glutathione, hydrogen peroxide, brain tissue