天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (4): 453-456.doi: 10.11958/20150003

• 实验研究 • 上一篇    下一篇

缺血后处理减轻大鼠肢体缺血再灌注后肺损伤的实验研究

李开济， 贺宝玲， 卢秋玲， 门秀丽， 赵利军△   

  1. 河北省唐山市， 华北理工大学基础医学院病理生理学系 （邮编063000）
  • 收稿日期:2015-08-24 修回日期:2015-12-03 出版日期:2016-04-15 发布日期:2016-05-20
  • 通讯作者: △通讯作者 E-mail:786411506@qq.com E-mail:786411506@qq.com
  • 作者简介:李开济 （1994）， 男， 临床医学专业本科生， 主要从事肢体缺血再灌注损伤机制与防治的研究
  • 基金资助:
    河北省卫生厅医学研究重点课题 （20130060）

Experimental studies of ischemia postconditioning alleviated lung injury after limb ischemia reperfusion in rats

LI Kaiji, HE Baoling, LU Qiuling, MEN Xiuli, ZHAO Lijun△   

  1. Department of Pathophysiology, Basic Medical College, North China University of Science and Technology, Hebei 063000, China
  • Received:2015-08-24 Revised:2015-12-03 Published:2016-04-15 Online:2016-05-20
  • Contact: △Corresponding Author E-mail： 786411506@qq.com E-mail:786411506@qq.com

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摘要： 摘要： 目的 观察缺血后处理 （I-postC） 对大鼠肢体缺血再灌注 （LIR） 后肺损伤的影响， 探讨缺血后处理的器官保护作用及可能机制。方法 Wistar 大鼠 24 只随机分为 3 组 （n=8）， 对照组 （Control 组）、 缺血再灌注组 （IR 组） 和缺血后处理组 （I-postC 组）。结扎大鼠双后肢根部以阻断血流 4 h， 后再恢复血流灌注 4 h， 制作大鼠 LIR 动物模型。 Control 组橡皮带松弛环绕双后肢不阻断血流， I-postC 组在血流再灌注前， 行重复 3 次的缺血 5 min-再灌注 5 min，再恢复 4 h 的血流再灌注。留取血液及肺组织标本， 测定各组动物动脉血气指标氧分压[p （O2 ） ]和二氧化碳分压 [p （CO2 ） ]， 检测血浆及肺组织的丙二醛 （MDA） 含量及黄嘌呤氧化酶 （XOD） 和超氧化物歧化酶 （SOD） 水平， 光镜及电镜下观察肺组织的病理形态学改变。结果 LIR 后 p （O2 ） 和 p （CO2 ） 明显降低， 血浆和肺组织中 SOD 活性明显降低，而 XOD、 MDA 明显增加 （P < 0.05）； 镜下可见肺间质内血管扩张充血， 中性粒细胞浸润， 血管周围间隙增大， 肺泡间隔增宽， 肺泡腔内有渗出液等损伤表现。与 IR 组比较， I-postC 组 p （O2 ） 和 p （CO2 ） 明显增加， 血浆及肺组织 SOD 活性升高； 而 XOD、 MDA 水平降低 （P < 0.05）； 镜下观察肺组织只可见轻度病理改变。结论 I-postC 可通过抑制脂质过氧化反应减轻大鼠 LIR 后肺损伤的程度。

关键词: 再灌注损伤, 肺, 氧化性应激, 丙二醛, 黄嘌呤氧化酶, 超氧化物歧化酶, 缺血后处理

Abstract: Abstract: Objective To observe the effects of ischemic postconditioning (I-postC) on lung injury after limb ischemia reperfusion (LIR) in rats, and to investigate the protective effect and the mechanisms. Methods Twenty-four Wistar rats were divided into three groups: control group (group Control), ischemia-reperfusion group (group IR) and ischemic postcondi⁃ tioning group (group I-postC). Referring to routine method in our department, the model rats underwent 4- hour ischemia and 4-hour reperfusion of hind limbs were made. In group Control, the rubber band around the limb was loose,which did not block the blood flow. Rats in group I-postC were given repeated 3 times of 5 min ischemia-5 min reperfusion, and then did perfusion 4 h before reperfusion. The blood and lung samples were collected for detecting arterial gas of partial pressure of oxygen [p(O2)] and partial pressure of carbon dioxide [p(CO2)]. The plasma and lung tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD) and xanthine oxidase (XOD) were detected. The morphological changes of lung tissue were ob⁃ served under light microscope and electron microscope. Results It was found that after suffering from ischemia-reperfu⁃ sion, levels of p(O2) and p(CO2) decreased significantly. The activity of SOD in plasma and lung tissues decreased, but XOD and MDA increased significantly (P < 0.05). With microscope, lung interstitial vascular dilation, infiltration of neutrophils, the width of the alveolar space, alveolar septal thickening and alveolar exudate were found. Compared with IR group, it was found that p(O2) and p(CO2) increased significantly in group I-postC. The activity of SOD in plasma and lung tissues in⁃ creased, but XOD and MDA decreased significantly(P < 0.05). The mild damage of pathological changes were found. Conclu⁃ sion Ischemic postconditioning can reduce the lung injury after limb ischemia reperfusion in rats, which may be related to the inhibition of lipid peroxidation.

Key words: reperfusion injury, lung, oxidative stress, malondialdehyde, xanthine oxidase, superoxide dismutase, ischemic postconditioning