天津医药 ›› 2016, Vol. 44 ›› Issue (6): 669-671.doi: 10.11958/20150374

• 细胞与分子生物学 • 上一篇    下一篇

脂多糖对小胶质细胞血管内皮生长因子表达的影响

曾勇, 吕靖芳, 郁龚杰, 高伟伟, 张建宁   

  1. 天津医科大学总医院神经外科, 天津市神经病学研究所
  • 收稿日期:2015-12-04 修回日期:2016-01-26 出版日期:2016-06-15 发布日期:2016-07-04
  • 通讯作者: 曾勇 E-mail:zeng1988yong@163.com
  • 基金资助:
    国家自然科学基金面上项目 (81271361); 国家自然科学基金重点项目 (81330029)

The effect of LPS on VEGF expression in microglia

ZENG Yong, LYU Jingfang, YU Gongjie, GAO Weiwei, ZHANG Jianning   

  1. Department of Neurosurgery, Tianjin Medical University General Hospital, Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin 300052, China
  • Received:2015-12-04 Revised:2016-01-26 Published:2016-06-15 Online:2016-07-04

摘要: 摘要: 目的 探讨脂多糖(LPS)是否能够通过低氧诱导因子-1α(HIF-1α)调节小胶质细胞血管内皮生长因子(VEGF) 的表达。方法 培养 BV2 小胶质细胞系, 分为 4 组: 对照组、 LPS (100 µg/L) 刺激组、 LRS 干预组 (LPS 100 µg/L及脂多糖拮抗剂 200 µg/L), HIF-1α抑制剂干预组 (LPS 100 µg/L 及 FM19G11 10 mmol/L)。分别采用免疫荧光、 Western blot、 ELISA 检测 VEGF 和 HIF-1α 的表达情况。结果 与对照组比较, LPS 可显著增加 BV2 细胞 VEGF 表达, 脂多糖拮抗剂可明显抑制 LPS 的这一作用。HIF-1α的表达在 LPS 刺激 8 h 后明显增加。HIF-1α抑制剂 FM19G11 可减少 LPS 引起的 BV2 细胞 VEGF 表达增加。结论 LPS 促进小胶质细胞 VEGF 的表达, HIF-1α参与这一调节过程。

关键词: 脂多糖类, 小神经胶质细胞, 血管内皮生长因子类, 缺氧诱导因子 1, α亚基

Abstract: Abstract: Objective To investigate whether lipopolysaccharide (LPS) can induce vascular endothelial cell growth factor(VEGF) expression in microglia regulated by hypoxia inducible factor-1α (HIF-1α). Methods The cultured BV2 cells were divided into four groups: control group, LPS (100 µg/L) simulated group, LPS (100 µg/L) + LPS antagonist (LRS, 200 µg/L)intervened group and LPS (100 µg/L) + HIF-1α inhibitors FM19G11 (10 mmol/L) intervened group. Immunofluorescence staining,Western blotting and ELISA were used to detect the expressions of VEGF and HIF-1α. Results Compared with the control group, the VEGF expression level was obvious high in LPS simulated group (P < 0.05). LRS inhibited this effect of LPS (P <0.05). The HIF-1α level was increased in LPS simulated group at 8 h post-injury (P < 0.05). FM19G11, the inhibitor of HIF- 1α reduced the expression of VEGF induced by LPS (P < 0.05). Conclusion LPS can up-regulate the expression of VEGF by HIF-1α.

Key words: lipopolysaccharides, microglia, vascular endothelial growth factors, hypoxia-inducible factor 1, alpha sub? unit