天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (7): 853-856.doi: 10.11958/20160275

• 实验研究 • 上一篇    下一篇

环烯醚萜对烟酰胺-链脲霉素诱导的2型糖尿病小鼠生精功能的影响

陈彤   

  1. 天津市天津医院泌尿外科（邮编 300211）
  • 收稿日期:2016-04-08 修回日期:2016-05-09 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 陈彤 E-mail:tianhechentong@163.com
  • 作者简介:陈彤（1969）， 男， 副主任医师， 主要从事泌尿外科疾病的诊断及治疗

Effects of Iridoid on Spermatogenesis Functions of Nicotinamide-Streptozotocin Induced Type-2 Diabetic Mice

CHEN Tong   

  1. Department ofUrology, Tianjin Hospital, Tianjin 300211, China
  • Received:2016-04-08 Revised:2016-05-09 Published:2016-07-15 Online:2016-07-15
  • Contact: CHEN Tong E-mail:tianhechentong@163.com

PDF (PC)

3827

摘要： 目的:　探讨环烯醚萜对烟酰胺-链脲霉素诱导的2型糖尿病小鼠生精功能的影响。方法: 在本实验研究中，56只雄性小鼠随机分为7组(n=8)：对照组；糖尿病小鼠组；糖尿病小鼠服用格列本脲(0.25mg/kg)治疗组；糖尿病小鼠接受环烯醚萜(20和40mg/kg)治疗组；正常小鼠接受环烯醚萜(20和40mg/kg)治疗组。糖尿病模型制作：由腹腔注射链脲霉素(65mg/kg)，15分钟后注射烟碱(120mg/kg)。然后，连续喂服格列本脲和环烯醚萜28天。最后一次治疗24小时后，提取血清样本、睾丸及附睾组织，测定血激素、观察睾丸病理切片、进行精子参数评估。结果: 糖尿病小鼠体重和睾丸质量，精子浓度和活力，血清LH、FSH和T水平明显下降(p < 0.05)。接受环烯醚萜治疗组小鼠前述下降的参数得到明显提升(p < 0.05)。结论: 结果表明，针对糖尿病小鼠应用环烯醚萜可降低氧化损伤的危重程度、有效提升其生育能力, 具有确切的临床应用价值。

关键词: 糖尿病, 2 型, 环烯醚萜类, 促黄体激素, 卵泡刺激素, 睾酮, 精子, 小鼠, 近交 C57BL, 抗氧化作用

Abstract: Abstract Objective： This study was aim to investigate the effects of iridoid on the spermatogenesis functions of type-2 diabetes induced by nicotinamide and streptozotocin. Methods：56 male C57BL mice were divided into seven groups (n=8) randomly: control; diabetic mice; diabetic mice administered glibenclamide (0.25 mg/kg); diabetic mice who received the iridoid(20 and 40mg/kg groups); and normal mice received iridoid(20 and 40mg/kg)groups. Diabetic model was induced by intraperitoneal?injection of streptozotocin (65 mg/kg) and nicotinamide (120 mg/kg). Glibenclamide and iridoid were orally feed for the next 28 days. 24 hours after the last treatment, blood samples, testis and the epididymis were obtained for testis, sperm analysis and hormonal tests. Results: Body and testicular weight, sperm count and viability, and LH, FSH, T levels were significantly decreased in diabetic group(p<0.05). The diabetic mice treated with 40 mg/kg of iridoid recovered from these reductions(p<0.05). Glibenclamide relieved hormonal and sperm reduction in diabetic mice group(p<0.05). Conclusions：These results suggests that the iridoid has anti-infertility effects in diabetic males mice.

Key words: diabetes mellitus, type 2, iridoides； luteinizing hormone, follicle stimulating hormone, testosterone, spermatozoa, mice, inbred C57BL, antioxidant