Abstract: Objective To acquire theoretical data for studying the mechanism of miR-126 in male patients with laryngeal cancer, and predict and analyze the target gene of miR-126. Methods The cancer Genome Atlas (TCGA) database was analyzed for specific effects of miR-126 on male patients with laryngeal cancer. And then the target gene of miR-126 was predicted by target gene databases of male patients with laryngeal cancer. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment was analyzed by DAVID database. Results The high expression of miR-126 was beneficial to increase the overall survival (OS) of patients with laryngeal cancer. And the higher expression of miR-126 was found in normal tissue. Seven of 58 target genes (DNMT1, GRIN2B, L2HGDH, PIK3R2, PTPN7, SIRT1, TWF1) worked in effecting the overall survival of patients with laryngeal cancer. GO enrichment analysis found that target genes constituted the heterochromatin, and involved in cell metabolism. KEGG pathway enrichment analysis found that PIK3R2 and SIRT1 took part in cell metabolism and apoptosis by AMPK and FoxO signal pathways. Conclusion MiR- 126 could improve the OS of male patients with laryngeal cancer, which is achieved by regulating gene expression, cell metabolism and apoptosis.

Key words: laryngeal neoplasms, miR-126, gene ontology, KEGG signal pathway enrichment