Tianjin Medical Journal

Tianjin Medical Journal ›› 2020, Vol. 48 ›› Issue (1): 1-7.doi: 10.11958/20192370

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The mechanism of PM2.5 induced lung epithelial cell BEAS-2B injury based on RNA-sequencing

HU Ling-juan1, HE Xiang2, ZHANG Lei2, RAN Qin2, LI Guo-ping1,2△   

  1. 1 Department of Clinical Medicine, Southwest Medical University, Luzhou 646000, China; 2 Department of Respiratory Medicine, Chengdu Third People’s Hospital, the Affiliated Hospital, Southwest Jiaotong University △Corresponding Author E-mail: lzlgp@163.com
  • Received:2019-08-13 Revised:2019-11-13 Published:2020-01-15 Online:2020-01-15

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Abstract: Objective To investigate the effect of PM2.5 on gene expressions of lung epithelial cell BEAS-2B and explore the signaling pathway. Methods BEAS-2B cells were divided into control group (PBS treatment) and experimental group (PM2.5 treatment). Total RNA of each group was extracted 24h after RNA-sequencing. After quality control, the sequence was compared with the reference genome. The mapped data (reads) were used for the assembly of subsequent transcripts, and the expression quantity calculation was obtained. And the results were analyzed in the following terms: NR, Swiss-Prot, Pfam, COG (Evolutionary genealogy of genes), GO (Gene Ontology), KEGG (Kyoto Encyclopedia of genes and genes) annotations in the six major databases. Differential expression genes (DEGs) were screened by DESeq2. Then, bioinformatics analysis was used to analyze the biological functions of DEGs, including GO and KEGG databases, and five key genes (FOSB, FOSL1, MUC5AC, CSF2 and IL-6) were detected by qRT-PCR for verification. Results The transcriptome data were compared between PM2.5 group and control group, and a total of 961 differentially expressed genes were obtained. Among them 453 genes were up-expressed and 508 genes were down-expressed. Through functional analysis of GO and KEGG, it was found that these differentially expressed genes were mainly involved in the regulation of protein phosphorylation, immune system process, positive regulation of signal transduction and apoptosis pathways. And they were significantly enriched in the IL-17 signaling pathway. qRT-PCR results showed that the relative expressions of key genes (FOSB, FOSL1 and IL-6) were significantly up-regulated in PM2.5 treatment group (P＜0.05), which was consistent with the results of RNA-seq. Conclusion It is possible that PM2.5 aggravates inflammatory response in beas-2b cells through regulating the key genes of the "IL-17 signaling pathway and promotes apoptosis.

Key words: RNA-seq, PM2.5, lung epithelial cell BEAS-2B, differential genes, IL-17 signaling pathway