Abstract:

Objective To investigate the expression and effect of pyrodeath related factors in decidual tissue of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) assisted early postpregnancy abortion. Methods A total of 102 patients with early abortion after IVF/ICSI assisted pregnancy were selected as the research group, and 100 patients who underwent artificial abortion due to unexpected pregnancy during the same period were selected as the control group. Samples of decidua tissue were collected. Quantitative real-time PCR (qRT-PCR) method and Western blot assay were used to detect expression levels of Nod-like receptor pyrin domain 3 (NLRP3), interleukin-1β (IL-1β), Cysteinyl aspartate specific proteinase 1 (Caspase1), gasdermin D (GSDMD) mRNA and protein in decidua tissue. TUNEL method was used to detect the cell pyroptosis in decidua tissue. Pearson method was used to analyze the correlation between pyroptosis rate and the protein levels of NLRP3, IL-1β, Caspase1 and GSDMD in decidua. Results Compared with the control group, expression levels of NLRP3, IL-1β, Caspase1, GSDMD mRNA and protein, the cell pyroptosis rate in decidua tissues were significantly increased in the research group (P<0.05). There was a positive correlation between cell pyroptosis rate in decidua tissue and expression levels of NLRP3, IL-1β, Caspase1 and GSDMD in the research group (r=0.429, 0.474, 0.498, 0.540, all P<0.05). Conclusion The expression levels of pyrolysis-related factors NLRP3, IL-1β, Caspase1 and GSDMD in decidua tissue are increased in patients with early abortion after IVF/ICSI assisted pregnancy, and which are related to the cell pyroptosis of decidua tissue.

Key words: abortion, therapeutic, fertilization in vitro, sperm injections, intracytoplasmic, decidua tissues, Nod-like receptor pyrin domain 3, interleukin-1β, cysteinyl aspartate specific proteinase 1, gasdermin D