Abstract: Objective: Implantation of auto-tissue engineering bone to cure the rabbit segmental bone defects. Methods:Culture the rabbit mesenchymal cell， prepare the auto-tissue engineering bone with CS-Gel/HA－rhBMP-2. Bone mesenchymal stem cells as seed cells and carry out complex CS-Gel/HA-rhBMP-2.The establishment of animal models of bone defect is to amputate forelimb mid-7mm length of the rabbit bone tissue . In experimental group（right side），treat the rabbit segmental bone defect model with CS-Gel/HA－rhBMP-2－seed cell,in control group (left side),the implantation is CS-Gel/HA.Not do internal and external fixation,sew up the wound layer by layer.Evaluate the grade of X-ray and histology after treatment 4,8,12 weeks.Results:There are no significant differences of X-ray grade and histology grade between experiment group and control group at 4 weeks after treatment, but the X-ray grade and histology grade is better in experiment group than in control group at 8,12weeks after treatment，data are statistically significant (p<0.05).Conclusion: The auto-tissue engineering bone can cure the rabbit segmental bone defects.

Key words: Bone mesenchymal cell, Auto-tissue engineering bone, Bone defect, Cure