Abstract: Abstract Objective:To investigate the feasibility of the small intestinal submucosa as a scaffold for tissue engineering tendon. Methods:The cells were seeded in the SIS. The adhesion of the cell was observed by the somatotype microscope.40 Roman chickens for 12weeks old were divided into two groups randomly, cell-seeded SIS group(experimental group) and autograft group(control group). The chickens were sacrificed at 3, 6, or 9 weeks after surgery. The specimens were observated including general appearance somato- typemicroscope, histologic pattern, and mechanical test. Results:The wound was healed primarily and the reactor was little in the experimental group after surgery. Histology shows few fibers and the confused alinement of fibers at the 3rd week. The increasing amount of fibers was seen at the 6th week, the alinement of fibers become a certain sequence. At the 9th week, a most sums of fibers, spread regularly,can be seen. Mechanical strength in the two groups have not statistical different significantly at the9thweek(P>0.05).Conclusion:Cell-seeded SIS scaffold may be the suitable substitution for the defected tendons, SIS perhaps becomes a ideal scaffold in the tissue engineering tendon.

Key words: Small-intestinal submucosa, tendon, Tissue engineering, Scaffold