Abstract: [Abstract] Objective To study the effects of improved Wright-Giemsa staining. Methods To evaluate the morphological characteristics of the sperm of 40 cases of semen by both traditional and improved Wright-Giemsa staining; and to assess the staining effects of improved Wright-Giemsa staining on vaginal discharge. Results The defective rates of the middle and tail parts of sperm are respectively 7.02± 2.4% and 11.02±2.03% in improved staining group; while that is 5.48±2.8% and 8.05±2.56% in traditional staining group. There is statistical significance between these staining methods (p<0.05). There is no significant difference of the staining of normal sperm and sperm head between these two staining methods (p<0.05); however, improved staining group shows a much clear nucleus and acrosome staining than traditional methods. Improved Wright-Giemsa staining is better for sperm, vaginal discharge and Trichomonas vaginalis. More importantly, the axostyle and flagella are clearly stained with the improved methods. Conclusion Improved Wright-Giemsa staining is simple and can be used in clinical diagnosis.

Key words: Improved Wright-Giemsa staining, Sperm, Vaginal discharge, Trichomonas vaginalis, staining effect