Tianjin Medical Journal

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The expression of the calcium-sensing receptor in human osteoarthritis cartilage

LIU Jun 1,CAO Jiangang 2,YAO Qingbin 3,LI Zhijun 2,FU Xin 4,MA Xinlong 5   

  1. 1. General Hospital of Tianjin Medical University, Tianjin 300052, China;
    2. General Hospital of Tianjin Medical University, Tianjin 300052, China
    3. Basic Medical College of Tianjin Medical University
    4. Department of Joint Surgery, Tianjin Hospital;
    5. General Hospital of Tianjin Medical University, Tianjin 300052, China;Department of Joint Surgery, Tianjin Hospital
  • Received:2013-12-24 Revised:2014-05-12 Published:2014-09-15 Online:2014-09-15
  • Contact: MA Xinlong

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Abstract:

[Abstract] Objective To investigate calcium-sensing receptor (CaSR) expression level in osteoarthritis (OA) carti?
lage，and the relationship with OA pathogenesis thereof. Methods The degenerated human cartilage of distal femur and
proximal tibia came from 5 patients with OA underwent total knee replacement surgery. The tissue specimens were stained
by HE. According to the Collins histological grading standards, articular cartilage degeneration was classificated in speci?
mens. Eight HE slices of Collins grade 0 were used as normal cartilage group. Eight HE slices of Collins gradeⅡwere used
as degeneration cartilage group. The CaSR expression levels detected by immunohistochemical staining technique were com?
pared between two groups. Results  Expression of CaSR was detected in osteoarthritis cartilage. The expression intensity of
CaSR was significantly lower in normal cartilage group than that of degeneration of cartilage group (score：1.63±0.95 vs 3.52±0.78，t=8.99，P＜0.05). Conclusion  The activation of CaSR is associated with the degeneration of articular cartilage.

Key words: osteoarthritis, knee, articular chondrocytes, calcium-sensing receptor, Immunohistochemistry