Abstract: Abstract Objective:To investigate the effect of thiazolidinediones on expression of chemerin,cmklr1 and tumor necrosis factor-alpha mRNA in liver of diabetic rat model.Methods:Murine diabetic models were induced with streptozotocin (STZ). Rats were randomized into normal control group，diabetes group and Pioglitazone group. After successful modeling,the Pioglitazone concentrations of 15 mg/d were given to rats of Pioglitazone group according to 1 kg of rat body weight.Rats were sacrificed after 8 weeks of experiments. At the same time, the liquid nitrogen frozen specimens from liver were taken to use to analyze expressions of chemerin、cmklr1 and tumor necrosis factor-alpha mRNA by the method of real-time quantitative PCR. Results: Results of real-time quantitative PCR showed that expressions of chemerin mRNA was significantly lower in diabetes group than that of normal control group and Pioglitazone group（P <0.017）.No significant difference was found in expressions of cmklr1 mRNA between three groups.The expression of TNF-α mRNA was higer than that of normal control group（P <0.017）. Conclusion:The expression of TNF-α mRNA was upregulated in liver of diabetes group. Pioglitazone may improving insulin sensitivity of liver by upregulating expression of chemerin mRNA

Key words: thiazolidinedionesdiabetes mellitus, experimentallivertumor necrosis factor-alpharats, Sprague-Dawleyreal-time quantitative PCR chemerin cmklr1, 实时定量聚合酶链反应