Abstract: Abstract Objective: Observe the effect of Tongjing Bushen recipe on oxidative stress of Alzheimer’s Disease model rats induced by Al. Methods: Chose 84 healthy SD rats, 3-month old, randomly divided them into Control Group(Group C), Model Group ( including low-dosed Al feed group—Group M1, middle-dosed Al feed group—Group M2 and high- dosed Al feed group—Group M3) and Chinese-medicine-recipe Group (including low-dosed group—Group N1, middle-dosed group—Group N2, high- dosed group—Group N3).There were 12 rats in each group. The rats in model groups were fed with conventional feed mixed with A1C13?6H2O.The rats were exposed to this feed for 3 months, and then made into AD animal model. Determine the learning and memory ability of rats with Y-electric. The escaping latent periods of rats in Group M were obviously prolonged, the escaping correct rates of rats in Group M obviously descended. The AD animal model was proved to be successful. The rats in Chinese medicine group were fed with high-dosed Al feed and fill the stomach by Tongjing Bushen recipe. After the experiment, the rats were taken blood from their right ventricles, serums were separated and kept in proper place. Took their brains quickly and made brain homogenate. Tested the activities of SOD and GSH-PX and the level of MDA in brain tissues and serum. Results: Compared with the rats in Group C, The activities of SOD and GSH-PX in the brain tissues and serum in the rats of Group M2 and Group M3 were strikingly decreased (P<0.01), the content of MDA was strikingly increased (P<0.01). Compared the rats in Group M1 with the rats in Group C and also compared the rats in Group M2 with the rats in Group M3, each index had no striking difference (P>0.05).Compared with the rats in Group M3, the activities of SOD and GSH-PX of the rats Group N2 and Group N3 strikingly increased (P<0.01), and the content of MDA strikingly decreased (P<0.01). Compared the rats in Group M3 with the rats in Group N1 and also compared the rats in Group N2 with the rats in Group N3, each index had no striking difference (P>0.05). Conclusion: The excessive intake of Al causes the rats to be in the state of intense OS. The Tongjing Bushen recipe strikingly increased the rats' antioxidant ability and protected their central nervous system.group, middle-dosed Al feed group and high- dosed Al feed group) and Chinese-medicine-recipe Group (including low-dosed group, middle-dosed group, high- dosed group).There were 12 rats in each group. The rats in model groups were fed with conventional feed mixed with A1C13?6H2O.The rats were exposed to this feed for 3 months, and then made into AD animal model. The rats in Chinese medicine group were fed with high-dosed Al feed and fill the stomach by Tongjing Bushen recipe. After the experiment, the rats were anesthetized, open their chests, took blood from their right ventricles, serums were separated and kept in proper place. Took their brains quickly and made brain homogenate. Tested the activities of SOD and GSH-PX and the level of MDA in brain tissues and serum. Results: Compared with the rats in Control Group, The activities of SOD and GSH-PX in the brain tissues and serum in the rats of middle- and high- dosed Al feed group were strikingly decreased (P<0.01), the content of MDA was strikingly increased (P<0.01). Compared with the rats in high-dosed Al feed groups, the activities of SOD and GSH-PX of the rats in model- and high- dosed Chinese medicine groups strikingly increased (P<0.01), and the content of MDA strikingly decreased (P<0.01) Conclusion: The excessive intake of Al causes the rats to be in the state of intense OS. The Tongjing Bushen recipe strikingly increased the rats' antioxidant ability and protected their central nervous system.

Key words: Tongjing Bushen Recipe, aluminum, Alzheimer’s disease, super oxide dismutase, glutathione peroxidase, malonaldehyde