Abstract: Abstract 0bjective: To investigate the value of serum AFP-L3 variants assay for differential diagnosis of liver disease.Methods : serum AFP-L3 variants were isolated by micro centrifugal Column,and the contents of AFP and AFP-L3 were detected by Elcteochemo luminescence immuno assay(ECLIA). Result：When AFP-L3/AFP＞10% was used as a positive criterion ,in the cases of AFP＞400ng/ml and AFP＜400ng/ml, the positive rate of AFP-L3 were 79.0% And 75.9%, respectively in hepatocellular carcinoma(HCC), which in liver cirrhosis(LC)、chronic hepatitis(CH) and autoimmune hepatitis(AIH) were 8.7%、7.9% and 7.3% ,respectively.Conclusion ：AFP-L3 is very useful in the differential diagnosis of HCC and low AFP level liver disease.

Key words: AFP, AFP-L3, micro centrifugal Column, ECLIA