Abstract: Objective To investigate the effect and regulation mechanism of mimic ischemia/reperfusion culture of human umbilical vein endothelial cells (HUVECs) on pro-inflammatory factors. Methods HUVECs were divided into 4 groups: control group(culture with normal media), mimic ischemia/reperfusion group(mimic ischemic media cultured for 30min followed by normal media cultured for 4h), normal culture +p65 siRNA group(HUVECs were transfected with p65 siRNA 48h before mimic ischemia/reperfusion), mimic ischemia/reperfusion group +p65 siRNA group. The mRNA and protein expressions of TNF-αand ICAM-1 in each group were determined by real-time PCR and enzyme linked immunosorbent assay (ELISA), respectively. Results The mRNA and protein levels of TNF-αand ICAM-1 were significantly increased by mimic ischemia/reperfusion (P < 0.05), which were otherwise significantly inhibited by the transfection of p65 siRNA(P < 0.05). Conclusions Silencing p65 through the transfection of p65 siRNA in HUVECs inhibited mimic ischemic/reperfusion- induced expression of TNF-αand ICAM-1.

Key words: siRNA, mimic ischemic/reperfusion, human umbilical vein endothelial cells, pro-inflammatory factors