Abstract: Objective: To detect the amount of VEGF-D (vascular endothelial growth factor-D ) and MLVD(micro-lymphatic vessel density) in the gastic cancer, analyze the relationship of VEGF-D and MLVD with the following factors: age, gender, clinical stage，lymph node metastasis，histo-differentiation and explore the use of antilymphangiogenic treatment. Methods: 52 samples of the gastric cancer tissue and bordeline tissue of cancer were gathered . 52 samples of normal tissue at least 5cm away from the tumor were also collected. We adopted an immunohistochemistry method to determine the levels of VEGF-D and MLVD in the samples. This was accomplished by bingding the clinical data and stastistical analysis to find how much VEGF-D and MLVD were present in the gastric cancer and normal tissue and the relationship with age, gender, clinical stage ，lymph node metastasis ，histo-differentiation . Results: The expressive rate of VEGF-D in gastric cancer and the normal tissue adjacent cancer had significantly difference (P<0.01 ). The positive rate of VEGF-D in gastric cancer in the samples which had lymph node metastasis was significantly higher than which had no lymph node metastasis（P<0.01 ). The positive rate of VEGF-D in gastric cancer of stage Ⅲ、Ⅳ was significantly higher than that in stage Ⅰ、Ⅱ（P<0.01）. The expression of VEGF-D had no significantly different from poor and well differentiation（P>0.05）. The expression of VEGF-D had significant correlation with lymph node metastasis ( P<0.01). The positive rate of VEGF-D was also correlated with the clinical stage（P<0.01）. The MLVD in the normal tissue was significantly higher than that in the gastric cancer ( P<0.01 ). The MLVD in tumor borderline was higher than that in the normal tissue and gastric cancer ( P<0.01). The MLVD average value in tumor borderline was significantly increased in the VEGF-D positive group than the negative group ( P<0.01). No significantly difference in the MLVD of VEGF-D positive group with the MLVD average in negative group（P>0.05）. Conclusion:The expression of VEGF-D in the gastric cancer is high, and it has correlation with clinical stage, lymph node metastasis. It suggests that the expression of VEGF-D is important in promoting tumor growth and metastasis. MLVD in the tumor borderline was significantly higher than in gastric cancer and normal tissue . The increase of MLVD may be related to the invasion and metastasis of gastric cancer in the tumor borderline. The detection of VEGF-D and MLVD in the tissue of gastric cancer can be accepted as important affiliated markers to evaluate the lymph node metastasis. It may prove to be markers to predict the malignant biology of gastric cancer and prognosis.