Abstract:

[Abstract] Objective To investigate the effects of MTA1knock down on migration and invasion of NPC cell5-8F in vitro. Methods RNAi (Si-MTA1-01and Si-MTA1-02) that can transiently silenced MTA1was designed, synthesized and transfected into5-8F cells by lipofectamine2000. Control group (transfection with nonsense sequence) was also estab? lished. The efficiency of MTA1depletion was determined by q-PCR and Western blot. Wound-healing assay ,Matrigel inva? sion assay and thesolid-phase adhesion assay were performed to investigate the effect of MTA1knockdown on5-8F cell me? tastasis. Results Transiently knock down of MTA1decreased MTA1transcription and expression in 5-8F cells compared to shRNA-con cells, showing by Real-time PCR and western blot. The invasion and migration of the cells transfected with siRNA-MTA1were much weaker than the control group (P<0.05). Conclusion silencing MTA1gene can effectively in? hibit the migration and invasion of nasopharyngeal carcinoma cell, and might be a promising target for NPC treatment.