Abstract: Abstract: Objective To investigate the influence of Glial Cell Line–Derived Neurotrophic Factor(GDNF) to the expression of Matrix Metalloproteinase 9(MMP-9) in pancreatic carcinoma. Methods Make use of RT-PCR 、Western blot and Elisa to investigate the effect of GDNF to the expression of MMP-9 mRNA and protein in MIA PaCa-2 and Capan-2 human pancreatic carcinoma cell line. Results We found that GDNF can up-regulate the expressions of MMP-9 mRNA and its protein in a dose-dependent manner in RT-PCR and Elisa experiments. Conclusions GDNF has the effect to stimulate the expression of MMP-9 and thereby strengthen the anility of invasion in panereatic carcinoma. Key Words: Pancreatic Carcinoma; GDNF; MMP-9; Expression

Key words: Pancreatic Carcinoma, GDNF, MMP-9, Expression