Abstract:

[Abstract] Objective  To study the antibechic, expectorant and antiasthmatic actions of Clausena Lansium (Lour.) Skeels (CLLSL) leaf extracts. Methods （1）Sixty mice were randomly divided into six groups: normal control group, pentoxyverine group, petroleum ether extraction group, dichloromethane extraction group, ethyl acetate extraction group and liquid extraction group. After corresponding drug administration for seven days, cough models were induced by concentrated ammonia in mice. The latent phase and the cough frequency per two minutes were recorded in mice.（2）Another sixty mice were randomly divided into six groups: normal control group, ammonium chloride group, petroleum ether extraction group, dichloromethane extraction group, ethyl acetate extraction group and liquid extraction group. After corresponding drug administration for seven days, the concentration of tracheal phenolsulfonphthalein was detected by 5% phenolsulfonphthalein solution intraperitoneal injection.（3）Seventy mice were randomly divided into seven groups: normal control group, model group, dexamethasone group, petroleum ether extraction group, dichloromethane extraction group, ethyl acetate extraction group and liquid extraction group. The asthmatic mouse models were established by ovalbumin（OVA）intraperitoneal injection at the 1th, 7th and 14th day, and atomization inhalation was given from 18-22 days. The asthmatic mice were treated with corresponding medicines respectively before atomization inhalation. The total leukocyte number and different cell counts in bronchoalveolar lavage fluid (BALF) were observed. Results  (1) Compared with normal control group, the latent phase was significantly longer and the cough frequency per two minutes was significantly reduced in petroleum ether extraction and dichloromethane extraction groups (P ＜0.01). (2) Compared with normal control group, dichloromethane extraction, ethyl acetate extraction and liquid extraction could significantly increase the concentration of phenolsulfonphthalein of mouse trachea (P ＜0.01). (3) Compared with model group, the total leukocyte number, eosinophile granulocyte count and neutrophil count in BALF of asthmatic mice were significantly decreased in petroleum ether extraction group (P ＜0.01). Conclusion The extracts of CLLSL have antibechic, expectorant and antiasthmatic effects.

Key words: Clausena lansium(Lour.) Skeels, antibechic, expectorant, antiasthmatic, active fraction