Abstract: 【Abstract】ObjectiveTo study the functions of miR-204 in BDNF/TrkB signaling and pathogenesis in the epilepsy model after the transfected with miR-204.Methods Primary hippocampal neurons were cultured in vitro after 7 days.Epilepsy model of hippocampal neurons were established by being exposed to Mg2+ free media for 3 hours.Construct miR-204 lentivirusvector.Detected by immunohistochemistry and patch clamp technique and Western Blot technique.Results BDNF/TrkB signaling can be activated by exogenous BDNF and is inhibited under epileptic condition. BDNF/TrkB signaling is inhibited by high expression of TrkB.T rather than TrkB.FL under epileptic condition.miR-204 can down-regulate expression of TrkB.T, so as to remove the inhibition of BDNF/TrkB signaling. Conclusion BDNF and mir-204 can improve the inhibiting condition of BDNF/TrkB signaling,and then playing an important role in alleviating epilepsy disease.

Key words: Epilepsy, Trkb, Hippocampal neurons, MiR-204, Western Blot