Abstract: Objective::To evaluate the the biological effects of the chitosan thermosensitive hydrogel on human gingival fibroblasts (HGFs) and provide information about local application of the chitosan thermosensitive hydrogel on periodontal diseases. Methods：Human gingival fibroblasts were cultured in medium containing the chitosan thermosensitive hydrogel at different concentrations，MTT colorimetric assay were used to evaluate the proliferation and ALP kit was used to evaluate the ALP activity of the GFCs. Results：On the early cultures ，there was no significant difference between the 4 groups and the negative control group and each different concentrations of the chitosan thermosensitive hydrogel about the promotion on GFCs growth and proliferation（P＞0.05）. After 48h, the promotion of each different concentrations was better than those of control group,through the statistic analysis,there is statistical difference between the 4 groups and the negative control group（P<0.05）,there is no statistical difference between the the 25 times group and the 0.2 times group, 5 times group and the 1 times group （P＞0.05）.There is statistical difference between the the 25 times group and the 5 times group after 48h（P<0.05）. Conclusion：The results suggest that the chitosan thermosensitive hydrogel could promote the proliferation of the GFCs, local application of the chitosan thermosensitive hydrogel for treating periodontal diseases is feasible.

Key words: Human gingival fibroblast, periodontal disease, thermosensitive hydrogel, chitosan