Abstract: [Abstract] Objective To explore the effect of schisandra chinensis fruit ethanol extract on nephrin and desmin ex?
pression in adriamycin(ADR) induced podocyte injuryin vitro. Methods Conditionally immortalized mouse podocytes were
treated with ADR for 24hin vitro, then the medium was changed to medium with SE （250mg/L）for24h. Podocytes were di?
vided into four groups: control group，model group, SE intervention group and SE group. The expression of nephrin in podo?
cytes was detected by immunofluorescence. Western Blot was employed to assess nephrin and desmin expression. Transcrip?
tion level of nephrin and desmin were determined by qRT-PCR. Results Nephrin expression was distributed along the cell
membrane in linear or granular pattern in control group and SE group. Fluorescence intensity in model group was lower than
that of control group SE group and SE intervention group. There was no significant difference of nephrin and desmin protein
and mRNA level between control group and SE group. Compared with the model group, protein and mRNA level of nephrin
was lower than that of control group and SE intervention group.The protein expression and mRNA transcription of desmin in
model group was higher than those in control group and SE intervention group (P<0.05). Conclusion SE（250mg/L）has
no harmful effect on the podocytesin vitro. SE can protect the podocytes from damage by adriamycin in vitro. SE not only upregulate the expression of nephrin, but also down-regulate of desmin expression.

Key words: schisandra chinensis, podocyte inju ry, vitro, adriamycin, nephrin, desmin