Abstract: Abstract Objective:To construct eukaryotic red fluorescent protein(RFP) expressing recombinant plasmids, pERFP-C1-hTudor-SN-SN(1~4), which contain SN((1~4) fragments of human Tudor-SN. Methods:The genes of Tudor-SN fragments were amplified by PCR from the recombinant pSG5-Tudor-SN-flag plasmid and inserted into pERFP-C1 fluorescent expressing vector with EcoRI and BamHI site. These recombinant pERFP-C1-hTudor-SN-SN(1~4) plasmids were transfected into HeLa cells and the expression of red fluorescent fusion proteins was examined by fluorescence microscope. Results:The SN(1~4) fragments of Tudor-SN were sequenced correctly and detected in the products of the restriction single/double enzyme digestion. The red fluorescent fusion proteins were observed in HeLa cell after the transfection. Conclusion:These recombinant eukaryotic plasmids of pERFP-C1-Tudor-SN(1~4) were constructed successfully and expressed effectively. Key words Human Tudor-SN protein pERFP-C1 Recombinant plasmid Fusion protein

Key words: Human Tudor-SN protein, pERFP-C1, Recombinant plasmid, Fusion protein