Abstract: [Abstract] Objective  To investigate the influence of chronic inflammation on the growth and angiogenesis in mouse transplanted melanoma. Methods Sixty inbred C57BL mice were randomly divided into three groups，control group (C)，inflammation + tumor group (I+T) and tumor group (T). The subcutaneous balloon was made on the mouse back, and the suspension of B16 melanoma cells was injected into the groin areas in both I+T group and T group. Either complete Freund adjuvant (CFA) or physiological saline was injected into the subcutaneous balloon of mice respectively. No intervention measures were taken in C group. Tumor tissues were stained with CD31 immunohistochemistry and the microvessel density (MVD) was counted. Enzyme-linked immunosorbent assays (ELISA) was used to detect the serum contents of interleukin (IL)-6, IL-17, IL-23 and vascular endothelial growth factor (VEGF) in three groups. Results  The tumor volumes and weights were significantly larger in I+T group than those in T group from 15 days to 27 days after model establishment (P < 0.05 orP < 0.01). The values of MVD in tumor tissues were significantly higher in I+T group than those in T group 9, 15, 21 and 27 days after model estab lishment (P < 0.01). The serum levels of IL-6, IL-17, IL-23 and VEGF were significantly higher in I+T group and T group than those of C group (P < 0.01). The serum levels of IL-6, IL-17, IL-23 and VEGF were significantly higher in I+T group than those of T group except for the 9-day after model establishment (P < 0.05 orP < 0.01). Conclusion The chronic inflammation induced by CFA can promote the development of mouse melanoma. And tumor angiogenesis could be promoted by some cytokines secreted by inflammatory cells. 

Key words: melanoma disease models, animal inflammation mice, inbred C57BL neovascularization, pathologic Vascular Endothelial Growth Factors interleukins, 病理性