Abstract: Abstract: Objective To explore the infection status of high risk human papilloma virus (hrHPV) and its effects and clinical significance on expression of cysteinyl aspartate specific protease 1 (Caspase- 1) and interleukin 1 β（IL-1β）in tissues of human cervical carcinoma. Methods A total of 102 patients with cervical carcinoma (cervical carcinoma group), 60 patients with cervical intraepithelial neoplasia (CIN group) and 30 patients with normal cervix (control group) were usedas the research objects. PCR reverse dot hybridization method combined with DNA chip technique were used to detect hrHPV. The expressions of Caspase- 1 and IL-1β were detected by immunohistochemical technique. Data were analyzed between hrHPV positive group and hrHPV negative group, between single type of hrHPV infection group and multiple type of hrHPV infection group. The relationship between caspase-1 and IL-1β expression and clinicopathological parameters in cervical carcinoma patients were observed. Results HrHPV infection was detected in 75 cases（73.5％）in cervical carcinoma group and 11 types of hrHPV were detected. In these 11 cases, single type and multiple type of hrHPV infection were 61 cases（81.3％）and 14 cases（18.7％ ) separately. HrHPV infection rate was much higher in cervical carcinoma group than those in CIN group and control group（36.7％ and 6.7％). Caspase-1 and IL-1β positive rates were significantly higher in cervical carcinoma group（61.8% and 51.0%） than those in control group（26.7% and 23.3%). The positive rate of Caspase-1 was significantly higher in cervical carcinoma group than that in CIN group（40.0%, all P＜0.01). The positive rates of Caspase-1 and IL-1β（77.8% and 74.1%）were higher in hrHPV DNA negative group than those in hrHPV DNA positive group（56.0% and 42.7%). There were no statistical differences in positive rates of Caspase-1 and IL-1β between single type of hrHPV infection group and multiple type of hrHPV infection group（P＞0.05). The difference of positive expressions of Caspase-1 and IL-1β were significantly related with cell differentiation, tumor size, lymphatic metastasis and clinical stage（P＜0.05 or P＜0.01). Conclusion There are single and multiple types of hrHPV infection in cervical carcinoma and the infection rate is high. HrHPVs may promote the progression of cervical carcinoma by restraining the expressions of Caspase-1 and IL-1β.

Key words: cervical carcinoma, human papilloma virus, cysteinyl aspartatespecific protease 1, interleukin 1β, inflammasome