天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (3): 290-293.doi: 10.11958/20150055

• 细胞与分子生物学 • 上一篇    下一篇

中等强度静磁场对THP-1细胞分泌炎症因子的影响

张同存1,郭志霞1,2,毛立斌1,2,王会琴1,2,张蕊1,2   

  1. 1 天津科技大学生物工程学院分子药理学和分子生物学研究室（邮编 300457）， 2工业发酵微生物教育部重点实验室
  • 收稿日期:2015-07-20 修回日期:2015-11-20 出版日期:2016-03-15 发布日期:2016-03-15
  • 通讯作者: 张同存 E-mail:1083719547@qq.com
  • 作者简介: 郭志霞(1989)， 女， 硕士在读， 主要从事关节炎和骨质疏松研究
  • 基金资助:
    国家自然科学基金资助项目（31171303， 31270837）；教育部长江学者和创新团队发展计划（IRT1166）

The effect of moderate static magnetic fields on secretion of pro-inflammatory factors in THP-1 cells

GUO Zhixia, MAO Libin, WANG Huiqin, ZHANG Rui, ZHANG Tongcun   

  1. 1 Molecular Pharmacology and Molecular Biology Research Laboratory, College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457,China; 2 Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education
  • Received:2015-07-20 Revised:2015-11-20 Published:2016-03-15 Online:2016-03-15
  • Contact: ZHANG Tongcun E-mail:1083719547@qq.com

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摘要： 目的 观察中等强度静磁场对人单核细胞白血病细胞 THP-1 增殖和炎症因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-8 分泌的影响。 方法 取对数生长期 THP-1 细胞， 分为对照组和磁场处理组， 利用 60 mT、200mT、400 mT 的静磁场分别作用细胞 18 h、24 h，、48 h 后， CCK-8 法检测细胞增殖情况。 另取细胞分为对照组、磁场处理组、脂多糖（LPS）活化组、LPS+磁场组。 利用 60 mT、200 mT、400 mT 的静磁场分别作用磁场处理组和 LPS+磁场组 18 h、24 h、48 h 后， 酶联免疫吸附试验（ELISA）检测各组 TNF-α、IL-6、IL-8 的水平。 结果 （1）与对照组相比， 3种强度磁场处理组对 THP-1 细胞增殖无明显影响（P > 0.05）。（2）各组 24 h 时 TNF-α、IL-6 水平较 18 h 升高， IL-8 则无明显变化。 而 48 h 与 24 h 相比较， TNF-α出现下降， IL-6 无明显变化， IL-8 出现升高。 3 个时点 LPS 活化组 TNF-α、IL-6、IL-8 水平较对照组及磁场处理组升高， 经过磁场处理后， LPS+磁场组 IL-6、IL-8、TNF-α水平均较 LPS活化组下降（P < 0.05）。 结论 静磁场对 THP-1 细胞释放炎症因子有一定抑制作用， 可为类风湿关节炎的治疗提供理论依据。

关键词: 类风湿关节炎, 静磁场, THP-1 细胞, 炎症因子, 脂多糖, 酶联免疫吸附试验, 肿瘤坏死因子-α, 白细胞介素-6；白细胞介素-8

Abstract: Abstract: Objective To investigate the effect of moderate static magnetic fields (SMF) on secretion of inflammatory factors tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8) in human monocytic leukemic cell line THP- 1. Methods THP- 1 cells at logarithmic phase were divided into control group and magnetic treatment group. CCK-8 method was used to detect cell proliferation after THP-1 cells were exposed to 60 mT, 200 mT and 400 mT static magnetic fields at 18, 24 and 48 h. Then THP-1 cells were divided into control group, magnetic treatment group, LPS activation group and LPS+SMF treatment group. When magnetic treatment group and LPS+SMF treatment group were exposed to SMF at 18, 24 and 48 h, the levels of the cytokines TNF-α, IL-6 and IL-8 were determined by ELISA. Results (1)60 mT, 200 mT and 400 mT SMF had no significant effects on cell proliferation in THP-1 cells (P > 0.05). (2)THP-1 cells secreted more TNF-α and IL-6 in 24 h than 18 h in every group, while IL-8 didn′t change. Compared with 24 h, the secretion of TNF-α decreased and IL-6 didn′t change, while IL-8 increased in 48 h. At three sampled time THP-1 cells of LPS activation group secreted more TNF-α, IL-6, IL-8 than those of control group and magnetic treatment group. After magnetic treatment THP-1 cells of LPS+SMF treatment group secreted less TNF-α, IL-6, IL-8 than those of LPS activation group (P < 0.05). Conclusion Static magnetic field may have some inhibitory effects on release of TNF-α, IL-6, IL-8 from THP 1 cells, which can provide basic data for the treatment of rheumatoid arthritis.

Key words: rheumatoid arthritis, static magnetic field, THP-1 cells, inflammatory factors, LPS, ELISA, TNF-α, IL-6, IL-8