天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (5): 577-581.doi: 10.11958/20150207

• 实验研究 • 上一篇    下一篇

24 h 快速眼动睡眠剥夺大鼠血清的蛋白质组学初步研究

郭兴道 1,2， 郜苗苗 1， 李婷婷 1,2， 张癸荣 1△   

  1. 1北京， 总后勤部卫生部药品仪器检验所 （邮编 100071）； 2河北张家口， 河北北方学院
  • 收稿日期:2015-10-09 修回日期:2015-11-26 出版日期:2016-05-15 发布日期:2016-05-18
  • 通讯作者: 通讯作者 E-mail:guirong_zhang@126.com E-mail:guoxingdao@126.com
  • 作者简介:郭兴道 （1988）， 男， 硕士， 主要从事药学相关研究
  • 基金资助:
    国家自然科学基金资助项目 （30901581）

A preliminary study on serum proteomics approach in rats of 24-h rapid eye movement sleep deprivation

GUO Xingdao1,2, GAO Miaomiao1, LI Tingting1,2, ZHANG Guirong1△   

  1. 1 Institute for Drug and Instrument Quality Control of Health Department GLD of PLA, Beijing 100071, China; 2 Hebei North University
  • Received:2015-10-09 Revised:2015-11-26 Published:2016-05-15 Online:2016-05-18
  • Contact: △Corresponding Author E-mail:guirong_zhang@126.com E-mail:guoxingdao@126.com

PDF (PC)

3825

摘要： 摘要： 目的 探讨睡眠剥夺大鼠血清蛋白质组中差异表达蛋白质及其意义。方法 采用改良多平台水环境法 建立 24 h 快速眼动睡眠剥夺大鼠模型。采用随机数字表法将大鼠均分为模型（M）组、 模型对照（MC）组、 空白对照 （BC） 组， 每组 8 只大鼠。观察大鼠体质量变化， 采用 Morris 水迷宫实验检测睡眠剥夺对大鼠学习记忆能力的影响， 并用双向电泳技术筛选血清差异蛋白， LC-MS/MS 技术鉴定差异蛋白。结果 BC 组和 MC 组大鼠建模前后体质量 差异均无统计学意义。M 组大鼠毛发光泽下降， 精神稍差， 较亢奋， 体质量在睡眠剥夺后显著降低。24 h 快速眼动 睡眠剥夺对大鼠学习记忆能力无显著性影响。各组之间蛋白质点数目差异无统计学意义。质谱鉴定出 4 种表达下 降的蛋白， 分别为 Serotransferrin； Glutathione peroxidase 3； Ig kappa chain C region, B allele； Collagen alpha-2(I) chain。 结论 短期睡眠剥夺对机体的损伤可能与铁离子代谢、 氧化应激和免疫功能等有关

关键词: 睡眠剥夺, 血清, 记忆, 学习, 蛋白质组学, 大鼠, Wistar

Abstract: Abstract:Objective To investigate the differential expressions of serum proteins by proteomics approach in rat model of sleep deprivation. Methods A rat model of 24-h rapid eye movement sleep deprivation was induced by MMPM. Twenty four rats were randomly and averagely divided into three groups, namely, model group (M), model control group (MC) and blank control group (BC). Changes of body mass in rats were observed. Morris water maze test was used to evaluate the effect of sleep deprivation on learning and memory ability. Serum proteins were separated by two-dimensional electrophoresis and identified by LC-MS/MS. Results There was no significant difference in rat body weight between BC group and MC group. After sleep deprivation, mental irritable, pelage dull and weight loss were found in M group, but no significant changes were found in learning and memory ability. There were no significant differences in the number of protein spots between three groups. Four proteins were down regulated: Serotransferrin, Glutathione peroxidase 3, Ig kappa chain C region, B allele and Collagen alpha- 2(I) chain. Conclusion The short term sleep deprivation may be related to iron metabolism, oxidative d

Key words: sleep deprivation, serum, memory, learning, proteomics, rats, Wistar