天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (9): 1112-1114.doi: 10.11958/20160003

• 临床研究 • 上一篇    下一篇

IL-10 基因多态性及血清水平与颅内动脉瘤发病的关系

邵得明 1， 邱虹 2， 于向东 2， 马向辉 1， 李云超 2， 王玉珏 2   

  1. 1 河北省迁安市人民医院神经外科（邮编 064400）；2开滦总医院神经外科
  • 收稿日期:2016-01-04 修回日期:2016-04-08 出版日期:2016-09-15 发布日期:2016-09-28
  • 作者简介: 邵得明（1979）， 男， 主治医师， 硕士研究生， 主要从事脑血管病的基础研究

The relationship between gene polymorphism and serum expression of interleukin-10 with intracranial aneurysm

SHAO Deming1, QIU Hong2, YU Xiangdong2, MA Xianghui1, LI Yunchao2, WANG Yujue2   

  1. 1 Department of Neurosurgery, Qian’ an People’ s Hospital, Qian’ an 064400, China; 2 Department of Neurosurgery, Kailuan General Hospital
  • Received:2016-01-04 Revised:2016-04-08 Published:2016-09-15 Online:2016-09-28

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摘要： 目的 分析白细胞介素（IL）-10 基因启动子区－ 1082G/A、－ 819C/T 单核苷酸多态性（SNP）及血清 IL-10 水平与颅内动脉瘤（IAs）发病的关系。 方法 运用 PCR 及 DNA 直接测序的方法， 检测 206 例 IAs 患者（IAs 组）和 187 例非 IAs 患者（对照组）的 IL-10 基因启动子区 SNP 位点， － 1082G/A、－ 819C/T 的基因型频率和等位基因频率， χ2检验分析 IAs 组和对照组之间的差异； 采用 ELISA 法检测血清中 IL-10 水平， t 检验分析 2 组之间的差异。 结果 IL-10 基因启动子区－ 1082G/A 位点的 GG 基因型和 GA＋ AA 基因型， 以及 G 等位基因和 A 等位基因频率比较， IAs 组较对照组 GA＋ AA 基因型以及 A 等位基因频率更高， 差异均有统计学意义（P < 0.01）， GA＋ AA 基因型（OR 值为 4.137， 95%CI 2.476~6.914）和 A 等位基因（OR 值为 3.368， 95%CI 2.476~4.583）携带者有更高的 IAs 发病风险； IL-10 基因启动子区－ 819C/T 位点的 CC 基因型和 CT＋ TT 基因型以及 C 等位基因和 T 等位基因频率比较， IAs 组较对照组 CT＋ TT 基因型以及 T 等位基因频率更高， 差异均有统计学意义（P < 0.01）， CT＋ TT 基因型（OR 值为 3.393， 95% CI 1.952~5.900）和 T 等位基因（OR 值为 3.764， 95%CI 2.730~5.192）携带者有更高的 IAs 发病风险。 IAs 组血清 IL- 10 水平低于对照组（P < 0.01）。 结论 IL-10 基因启动子区 SNP 影响 IL-10 表达，IL-10 基因启动子区－ 1082G/A、－ 819C/T 多态性与 IAs 的发病有关。

关键词: 颅内动脉瘤, 白细胞介素 10, 单核苷酸多态性

Abstract: Objective To analyze the associations of the interleukin- 10 (IL- 10) promoter- 1082G/A and - 819C/T single nucleotide polymorphism (SNP) and serum level of IL- 10 with intracranial aneurysm (IAs). Methods The polymerase chain reaction (PCR) and DNA direct sequencing methods were used to detect IL-10 gene promoter district two SNP site, － 1082G/A and -819C/T genotype frequency and allele frequency in 206 patients with IAs and 187 controls. Chisquare test was used to analyze differences between two groups. The serum level of IL- 10 was analyzed by ELISA, and ttest was used to analyze significant differences between two groups. Results There were significant differences in genotypes of GG and GA+AA, as well as the alleles G and A, in -1082G/A locus between IAs group and control group (P＜ 0.01). There were higher frequencies of genotype GA+AA and the allele A in IAs group than those in control group (P＜ 0.01). There was higher risk of suffering IAs in patients with genotype GA+AA (OR=4.137, 95%CI: 2.476-6.914) and the allele A (OR=3.368, 95%CI: 2.476-4.583). There were higher frequencies of the genotype CT+TT and the allele T in -819C/ T locus in IAs group than those of control group (P＜ 0.01). There was higher risk of suffering IAs in patients with genotype CT＋ TT (OR=3.393, 95%CI: 1.952 -5.900) and the allele T (OR=3.764, 95%CI: 2.730-5.192). The serum level of IL-10 was significantly lower in IAs group than that of control group (P＜ 0.01). Conclusion The IL-10 promoter SNP influences the expression of IL-10. IL-10 promoter -1082G/A and -819C/T polymorphisms are correlated with the formation of IAs.

Key words: intracranial aneurysms, interleukin-10, single nucleotide polymorphism