天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (11): 1356-1358.doi: 10.11958/20160102

• 实验研究 • 上一篇    下一篇

甘草酸二铵干预兔基底动脉瘤形成的实验研究

桂铮， 汪子文， 张鹏飞， 赵文可， 于耀宇△   

  1. 武警后勤学院附属医院神经外科 （邮编 300162）
  • 收稿日期:2016-03-03 修回日期:2016-08-26 出版日期:2016-11-15 发布日期:2016-11-15
  • 通讯作者: △通讯作者 E-mail： yuyaoyu666@aliyun.com E-mail:yuyaoyu666@aliyun.com
  • 作者简介:桂铮 （1989）， 男， 硕士， 初级职称， 主要从事神经介入临床及基础研究
  • 基金资助:
    武警后勤学院附属医院基金重点项目 （FYZ201507）

The intervention effect of diammonium glycyrrhizinate on the formation of basilar artery aneurysm in rabbits

GUI Zheng, WANG Ziwen, ZHANG Pengfei, ZHAO Wenke, YU Yaoyu△   

  1. Department of Neurosurgery, the Affiliated Hospital, Logistics College, Armed Police Command, Tianjin 300162, China
  • Received:2016-03-03 Revised:2016-08-26 Published:2016-11-15 Online:2016-11-15
  • Contact: △Corresponding Author E-mail： yuyaoyu666@aliyun.com E-mail:yuyaoyu666@aliyun.com

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摘要： 目的 探讨甘草酸二铵 （DG） 干预兔基底动脉瘤发生的作用机制。方法 新西兰白兔 40 只， 按照随机数 字表法分为对照组、 动脉瘤 （IA） 组、 生理盐水 （NS） 组、 DG 组， 每组 10 只。其中对照组不做任何处理， 其余各组结扎 双侧颈总动脉建立基底动脉瘤形成模型。在造模后的第 1~7 天， IA 组不做处理， DG 组给予 DG ［20 mg/ （kg·d）］ 静脉 注射， NS 组注入等剂量生理盐水。使用免疫组化染色方法检测基底动脉尖核转录因子核因子 kappa B（NF-κB）、 基 质金属蛋白酶 9 （MMP-9） 表达情况， 使用免疫荧光染色方法检测基底动脉尖 α-平滑肌细胞肌动蛋白 （α-SMA） 表达 情况。结果 IA 组和 NS 组 NF-κB 和 MMP-9 表达均高于对照组， α-SMA 表达量低于对照组 （P＜0.05）； IA 组和 NS 组差异无统计学意义（P＞0.05）。DG 组 NF-κB 和 MMP-9 表达低于 IA 组和 NS 组（P＜0.05）， 而 α-SMA 表达量高 于 IA 组和 NS 组 （P＜0.05）。结论 DG 可抑制 NF-κB 和 MMP-9 的表达， 减轻血管壁内炎症反应， 抑制颅内动脉瘤 的形成。

关键词: 颅内动脉瘤, 炎症, NF-κB, 基质金属蛋白酶 9, 肌动蛋白类, 血管壁破坏性重构

Abstract: Objective To explore the mechanism and intervention effects of diammonium glycyrrhizinate (DG) on the formation of intracranial aneurysm (IA) in rabbits. Methods Forty New Zealand rabbits were randomly divided into four groups: control group, IA group, normal saline (NS) group and DG group, 10 rabbits for each group. Except for the control group, rabbits in other three groups were operated with bilateral common carotid artery ligation to produce basilar artery aneurysm formation model. DG group was injected intravenously with DG［20 mg/(kg·d)］ from the first day to the seventh day after operation, NS group was given same volume of normal saline, while IA group was injected nothing. The expressions of NF-κB and MMP-9 were detected by immunohistochemical staining, while α-SMA was stained with immunofluorescence. Results The expressions of NF- κB and MMP- 9 were significantly higher in IA group and NS group than those of control group, while α-SMA expression was significantly lower than that of control group (P < 0.01). There were no significant differences in expressions of NF-κB and MMP-9 between IA group and NS group (P＞ 0.05). The expressions of NF-κB and MMP-9 were significantly lower in DG group than those of IA group and NS group (P＜ 0.05). The expression of α- SMA was significantly higher in DG group than that of IA group and NS group (P＜0.05). Conclusion DG can downregulate the expression levels of NF- κB and MMP- 9 in intracranial aneurysm, suppress inflammatory in artery wall and reduce the pathological changes and have intervention effect on intracranial aneurysm formation.

Key words: intracranial aneurysm, inflammation, NF- kappa B, matrix metalloproteinase 9, actins, destructive remodeling of vascular wall