天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (7): 839-842.doi: 10.11958/20160105

• 细胞与分子生物学 • 上一篇    下一篇

YY1基因在肺癌组织中的表达及其对肺癌细胞功能的影响

王玉 刘奔 曲金力 俞晓 冯楠楠 钱碧云   

  1. 1 天津医科大学肿瘤医院， 肿瘤研究所肿瘤分子流行病与生物统计研究室， 国家肿瘤临床研究中心， 天津市肿瘤防治重点实验室（邮编 300060）；2天津市西青区疾病预防控制中心；3 上海交通大学公共卫生学院
  • 收稿日期:2016-03-03 修回日期:2016-04-18 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 王玉 E-mail:yuwang9113@163.com
  • 作者简介:王玉（1991）， 女， 硕士在读， 主要从事肿瘤分子流行病学研究
  • 基金资助:
    国家自然科学基金资助项目（81573231）；天津市自然科学基金资助项目（13JCYBJC23100）

Expression of YY1 in lung cancer and its function on lung cancer cell lines

WANG Yu, LIU Ben, QU Jinli, YU Xiao, FENG Nannan, QIAN Biyun   

  1. 1 Department of Epidemiology and Biostatistics, Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center of Cancer,Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China; 2 Xiqing District Center for Disease Control and Prevention, Tianjin；3 School of Public Health, Shanghai Jiao Tong University
  • Received:2016-03-03 Revised:2016-04-18 Published:2016-07-15 Online:2016-07-15
  • Contact: WANG Yu E-mail:yuwang9113@163.com

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摘要： 目的 检测转录因子YY1在肺癌组织中的表达情况，并分析其对肺癌细胞的增殖、迁移及侵袭的影响。方法 应用Taqman探针实时荧光定量PCR方法检测235例肺癌组织以及62例配对癌旁组织中YY1 mRNA的表达，并分析其表达与患者临床病理特征的关系。应用Lipofectamine 3000试剂在肺癌细胞系转染YY1过表达质粒及对照质粒。噻唑蓝（MTT）分别检测对照组和YY1过表达组在24、48及72 h对A549和H1299细胞增殖的影响。划痕实验和Transwell实验检测YY1过表达对肺癌细胞的迁移和侵袭能力的影响。结果 与癌旁组织（5.80±0.58）相比，YY1 mRNA在肺癌组织（5.07±0.58）中表达下调（P<0.01），且YY1 mRNA的表达在不同性别、吸烟状态、病理类型及临床分期中存在差异（P<0.05）。YY1过表达组H1299细胞增殖能力在48h和72h均低于对照组（P<0.01），A549细胞在72h低于对照组（P<0.05）。划痕实验和Transwell实验结果显示转染过表达YY1质粒后，肺癌细胞的侵袭能力及迁移能力明显下降（P<0.05）。结论 YY1在肺癌组织中低表达，上调 YY1的表达可抑制肺癌细胞的增殖、迁移和侵袭。

关键词: 关键词:肺肿瘤, YY1转录因子, 细胞增殖, 细胞运动, 侵袭

Abstract: Abstract：ObjectiveTo investigate the gene of YY1 in lung cancer tissues and discuss its function in cell proliferation，migration and invasion.Methods The mRNA of YY1 in 235 lung cancer tissues and 62 adjacent tumor tissues were determined by real-time quantitative PCR. NSCLC cell lines were transfected with YY1 over- vector and negative control vector using Lipofectamine 3000. Proliferation of cells were measured by MTT in control groups and YY1 over- groups after 24h, 48h and 72h of transfection in A549 and H1299 cells. Wound-healing assay and Transwell assay were performed to investigate the effect of YY1 over on the invasion and migration of lung cancer cells.Results Compared to normal adjacent tissues(5.80±0.58)，the of YY1 was significantly lower in lung cancer tissues(5.07±0.58)(P<0.01)．There were significant differences in the levels of YY1mRNAbetween gender, smoking status,histology subtypeor clinical stages(P＜0.05). Furthermore, compared to controls, the over- ofYY1 in H1299 cells was found to suppress proliferation for 48h and 72h (P<0.01) and A549 cell for 72h(P<0.05). Wound-healing assay and Transwell assay showed the over of YY1 inhibited migratory and invasive capability of lung cancer cells (P<0.05).Conclusion YY1mRNA level is lower in lung cancer tissue than normal adjacent tissue and YY1 suppresses lung cancer cell proliferation, migration and invasion.

Key words: lung cancer, YY1transcription factor, cell proliferation, cell migration, invasive ability