天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (10): 1221-1224.doi: 10.11958/20160290

• 实验研究 • 上一篇    下一篇

香叶醇对大鼠颈动脉球囊损伤后血管内膜增生的抑制作用

江金垚,徐西子   

  1. 天津医科大学第二医院心脏科（邮编 300211）
  • 收稿日期:2016-04-12 修回日期:2016-06-06 出版日期:2016-10-15 发布日期:2016-10-21
  • 通讯作者: 江金垚 E-mail:jiangjy963@126.com
  • 作者简介: 江金垚（1987）， 男， 硕士， 主要从事冠心病基础研究

Geraniol attenuates vascular smooth muscle cell proliferation in rat carotid artery balloon injury model

JIANG Jinyao, XU Xizi#br# #br#   

  1. Department of Cardiology, the Second Hospital of Tianjin Medical University, Tianjin 300211, China
  • Received:2016-04-12 Revised:2016-06-06 Published:2016-10-15 Online:2016-10-21
  • Contact: JIANG Jinyao E-mail:jiangjy963@126.com

PDF (PC)

3685

摘要： 目的 研究香叶醇对球囊损伤大鼠颈动脉损伤后血管内膜新生的影响及可能的机制。 方法 20 只雄性成年 SD 大鼠， 随机均分为 4 组。（1）假手术组： 不予以球囊损伤。（2）对照组： 予以球囊损伤， 不予以药物干预。（3）低浓度组： 球囊损伤后每日口服香叶醇 50 mg/kg。（4）高浓度组： 球囊损伤后每日口服香叶醇 200 mg/kg。 制作球囊损伤模型， 2 周后留取球囊损伤段血管制作病理切片， 苏木精-伊红（HE）染色法测量内膜与中膜（I/M）比值， 免疫组化法检测增殖细胞核抗原（PCNA）的表达， 用积分光密度（IOD）值表示， 并测定组织中氧化应激标志物 8-羟基脱氧鸟苷（8-OHdG）和脂质氧化产物丙二醛（MDA）的水平。 结果 与假手术组比较， 对照组 I/M 比值、IOD 值、8-OHdG 及 MDA 水平均升高； 与对照组比较， 低浓度组 I/M 比值、IOD 值、8-OHdG 均降低， 但 MDA 水平与对照组差异无统计学意义。 高浓度组与对照组比较 I/M 比值、IOD 值、8-OHdG 及 MDA 水平均降低， 且较低浓度降低更明显（P < O.05）。结论 香叶醇抑制大鼠颈动脉球囊损伤后内膜增生， 其机制可能与下调 PCNA 蛋白表达， 抑制活性氧的产生有关。

关键词: 颈动脉损伤, 血管内膜, 氧化性应激, 大鼠, Sprague-Dawley, 疾病模型, 动物, 香叶醇

Abstract: Objective To evaluate the effects of geraniol(GOH) on neointima hyperplasia in rat carotid artery balloon injury model, and explore the potential molecular mechanisms associated with this effect. Methods Totally 20 male Sprague-Dawley (SD) rats were randomly divided into sham operation group (without balloon injury), control group (with balloon injury), low concentration group (with 50 mg/kg GOH intervention after balloon injury) and high concentration group (with 200 mg/kg GOH intervention after balloon injury). The intima to media (I/M) area ratio of neointima was measured by hematoxylin- eosin (HE) staining. The expression of proliferating cell nuclear antigen (PCNA) was measured by immunohistochemical staining at 14th day after operation. As the marker of oxidative stress, the levels of 8-hydroxy- 2’ - deoxyguanosine (8-OHdG) and malondialdehyde (MDA) were measured by enzyme linked inmmnosorbent assay (ELISA). Results The I/M ratio, IOD, 8-OHdG and MDA values were increased in control group compared with those of sham group. The I/M ratio, IOD and 8-OHdG values were reduced in low concentration group compared with those of control group. But there was no significant difference in MDA level between low concentration group and control group. The I/M ratio, IOD, 8- OHdG and MDA values were significantly reduced in high concentration group compared with those of control group, which showed a more significant inhibitory effect than that of low concentration group (P＜ 0.05). Conclusion GOH could attenuate balloon iniury induced neointima hyperplasia, which might be related to its effect on inhibiting expression of PCNA and decreasing oxidative stress.

Key words: carotid artery injuries, tunica intima, oxidative stress, rats, Sprague-Dawley, disease models, animal, Geraniol