天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (11): 1343-1346.doi: 10.11958/20160545

• 实验研究 • 上一篇    下一篇

香烟烟雾染毒对雄性大鼠睾丸组织 ATP 酶活性的影响

张静， 仲春雪， 黄云飞， 张晨△， 何丽娟， 邹莹   

  1. 乌鲁木齐,新疆医科大学公共卫生学院毒理学教研室 （邮编 830011）
  • 收稿日期:2016-06-13 修回日期:2016-09-10 出版日期:2016-11-15 发布日期:2016-11-15
  • 通讯作者: △通讯作者 E-mail: zhangchendr@163.com E-mail:532130800@qq.com
  • 作者简介:张静 （1979）， 女， 副教授， 博士， 主要从事生殖发育毒理方向研究
  • 基金资助:
    国家自然科学基金资助项目 （81260433）； 新疆维吾尔自治区自然科学基金资助项目 （2014211C015）

The effect of cigarette smoke exposure on ATPase enzyme activity in testis of male rats

ZHANG Jing, ZHONG Chunxue, HUANG Yunfei, ZHANG Chen△, HE Lijuan, ZOU Ying   

  1. Department of Toxicology, College of Public Health, Xinjiang Medical University, Urumqi 830011, China
  • Received:2016-06-13 Revised:2016-09-10 Published:2016-11-15 Online:2016-11-15
  • Contact: △Corresponding Author E-mail:zhangchendr@163.com E-mail:532130800@qq.com

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摘要： 目的 探讨香烟烟雾染毒对大鼠睾丸结构及睾丸组织 ATP 酶活性的影响。方法 清洁级成年雄性 SD 大 鼠 70 只， 随机分为对照组 （10 只） 和低、 中、 高剂量染毒组 （每组 20 只， 分别给予 10 支、 20 支、 30 支香烟烟雾暴露）。 染毒组采用呼吸道静式染毒， 每日 1 次， 每次 30 min， 各剂量染毒组分别染毒 6 周和 12 周（每个时段 10 只大鼠）。 实验期间记录各组大鼠体质量变化。实验结束后， 摘取睾丸， HE 染色观察睾丸组织结构， 酶联免疫吸附试验检测睾 丸组织 Na＋-K＋-ATP 酶、 Ca２＋-ATP 酶活性。结果 随染毒剂量增加， 各组大鼠体质量逐渐降低 （P < 0.05）。染毒组 大鼠睾丸病理切片观察到不同程度损伤， 且随着染毒时间的延长和染毒剂量的增加， 损伤逐渐加重。染毒 6 周后， 高剂量组大鼠睾丸组织 Na+-K+-ATP 酶活性低于其他 3 组 （P < 0.05）， Ca2+-ATP 酶活性低于对照组 （P＜0.05）。染毒 12 周后， 低、 中、 高剂量组大鼠睾丸组织 Na+-K+-ATP 酶、 Ca2+-ATP 酶活性均较对照组显著降低（P < 0.05）。结论 香烟烟雾染毒可减缓雄性大鼠生长， 导致睾丸组织损伤， Na+-K+-ATP 酶和 Ca2+-ATP 酶活性降低。

关键词: 吸烟, 烟草, 烟雾, 大鼠, Sprague-Dawley, 睾丸, 腺苷三磷酸酶类, 钠钾交换 ATP 酶, 钙转运 ATP 酶类

Abstract: Objective To explore the effect of cigarette smoke exposure on testis structure and ATPase enzyme activity in testis of rats. Methods Seventy adult male SD rats were randomly divided into the control group (10 rats) and the low, medium, high- dose groups (20 rats in each group, which received the exposure of 10, 20, 30 sticks of cigarettes respectively). The experimental group was exposed to cigarette smoke for 30 min per day. Each dose group was exposed for six weeks and twelve weeks (10 rats each time). The body weights in each group were recorded during the experiment. At the end of the experiment, testes of rats were removed, and the testicular tissue structure was observed by HE staining. Na +-K +- ATPase enzyme and Ca2+-ATPase enzyme activity of testicular tissue of rats were detected by enzyme-linked immunosorbent assay. Results With the increasing of exposure doses, the body weights of rats decreased (P < 0.05). The microstructure changes of testis were observed in the experimental group, and with the increasing of exposure time and exposure dose, the damage was gradually worse. After 6- week exposure, the Na +- K +- ATPase enzyme activity of testicular tissue was significantly lower in high dose group than that of rest of three groups (P < 0.05), and the Ca2 +-ATP enzyme activity was significantly lower than that of the control group (P < 0.05). The activities of Na +-K +-ATPase enzyme and Ca2 +-ATPase enzyme in testicular tissue were significantly decreased in the low, medium and high-dose groups than those of control group after being exposed for 12 weeks (P < 0.05). Conclusion Cigarette smoke exposure can retard the weight gain, cause the testis tissue damaged and decrease the activities of Na+-K +-ATPase enzyme and Ca2+-ATPase enzyme in testis of male rats.

Key words: smoking, tobacco, amog, rats, Sprague- Dawley, testis, adenosine triphosphatases, sodium- potassiumexchanging ATPase, calcium-transporting ATPases